Constraints in T‐B cooperation related to epitope topology on E. coli β‐galactosidase. I. The fine specificity of T cells dictates the fine specificity of antibodies directed to conformation‐ dependent determinants
Constraints in T‐B cooperation related to epitope topology on E. coli β‐galactosidase. I. The fine specificity of T cells dictates the fine specificity of antibodies directed to conformation‐ dependent determinants
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T-B 合作的限制与大肠杆菌 β-半乳糖苷酶 I 上的表位拓扑相关。T 细胞的精细特异性决定了针对构象依赖性决定簇的抗体的精细特异性。
DOI：
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发表时间：
1985
影响因子：
5.4
通讯作者：
F. Celada
中科院分区：
文献类型：
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作者：
F. Manca;A. Kunkl;D. Fenoglio;Andrée Fowler;E. Sercarz;F. Celada
测试T和B细胞协同产生抗体时识别的蛋白质抗原表位之间的关系的实验不能仅仅依赖于半抗原载体模型。在目前的工作中，我们使用了大肠杆菌β-半乳糖苷酶作为模式抗原，这是一个已经很好地表征了其三级和四级表位的分子。T辅助细胞是通过用完整或变性的分子或几种β-半乳糖苷酶溴化氰肽刺激小鼠来培养的。在一系列体外辅助T细胞检测中，我们用天然抗原预免疫的B细胞面对不同的T细胞群，并测试了它们帮助产生(A)结合抗体和(B)针对单个构象表位的抗体的能力，其特征是保护酶免受热变性或激活缺陷的β-半乳糖苷酶的能力。根据我们的结果，(A)用天然或变性抗原启动的辅助性T细胞可以提供等量的T细胞帮助，甚至可以产生“构象”抗体；(B)其中一种多肽(CB-18)在提高结合抗体的帮助方面是最有效的；以及(C)在启动辅助性T细胞方面，两种多肽(CB-20和CB-21)分别在平均产生保护抗体和激活抗体方面排名最高。因此，并不是每个β-半乳糖苷酶特异的T辅助细胞都有助于为分子上任何特定表位特异的B细胞提供帮助，但存在优先配对，可能受邻近规则的支配。
Experiments to test the relationship between the epitopes on a protein antigen recognized by T and B cells in their collaboration to produce antibody cannot rely solely on hapten‐carrier models. In the present work we used E. coli β‐galactosidase, a molecule whose tertiary and quaternary epitopes have been well characterized, as the model antigen. T helper cells were raised by stimulating mice with the intact or the denatured molecule or with any of several β‐galactosidase cyanogen bromide pep‐ tides. In a series of in vitro helper T cell assays we confronted the various T popula tions with B cells preimmunized with the native antigen, and we tested their capacity to help production of (a) binding antibodies and (b) antibodies directed to single conformational epitopes, characterized by their capacity to protect the enzyme from heat denaturation or to activate defective P‐galactosidase. According to our results, (a) equivalent T cell help can be provided by T helper cells primed with native or denatured antigen, even for the production of “conformational” antibodies; (b) one of the peptides (CB‐18) is most efficient in raising help for binding antibodies; and (c) two peptides (CB‐20 and CB‐21) rank highest in priming T helper cells for the even‐ tual production of protecting and activating antibodies, respectively. Thus, not every β‐galactosidase‐specific T helper cell is useful in providing help to B cells specific for any particular epitope on the molecule, but rather preferential pairings exist, possibly governed by a proximity rule.
影响因子：
4.4
作者：
R. Chesnut;H. Grey
通讯作者：
R. Chesnut;H. Grey
T 细胞有助于细胞毒性 T 淋巴细胞反应。
影响因子：
6.2
作者：
Livnat,S;Corley,RB
通讯作者：
Corley,RB