Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression
Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression
复制标题
烟草生长素诱导基因的启动子可导致生长素诱导和根尖特异性表达
作者：
E. Zaal;F. Droog;C. Boot;L. Hensgens;J. Hoge;R. Schilperoort;K. Libbenga
在以前的研究中，我们已经确定了几个在添加2，4-二氯苯氧乙酸(2，4-D)后积累的mRNAs[45，46]。与cDNAs克隆pCNT103相对应的mRNAs被发现在生长素处理引起的细胞分裂反应之前短暂积累。在本研究中，我们测定了三个103类基因和两个103类基因GNT1和GNT35的序列。为了进一步研究这些基因的表达调控，将它们的5‘端与β-D-葡萄糖醛酸酶报告基因(GUS)进行了翻译融合。GNT1 5‘区只在转基因植株的根尖表达。利用转基因毛状根培养物和转化细胞悬浮培养物表明，GNT1和GNT35的5‘端区域均可诱导2，4-D诱导表达GUS活性。对103类基因与其他生长素调控基因的同源性进行了评估。
In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells [45, 46]. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we determined the sequences of three 103-like cDNAs and two 103-like genes, GNT1 and GNT35. To further study the regulation of the expression of these genes their 5′ regions were translationally fused with the β-D-glucuronidase reporter gene (GUS). The GNT1 5′ region led to GUS expression only in the root tips of transgenic plants. By using transgenic hairy-root cultures and transformed cell suspension cultures it was shown that the 5′ regions of both GNT1 and GNT35 lead to 2,4-D-inducible expression of GUS activity. The homology of the 103-like genes with other auxin-regulated genes is evaluated.
影响因子：
11.6
作者：
MCCLURE, BA;HAGEN, G;GUILFOYLE, TJ
通讯作者：
GUILFOYLE, TJ