Oncogenic heterogeneous nuclear ribonucleoprotein D-like promotes the growth of human colon cancer SW620 cells via its regulation of cell-cycle
Oncogenic heterogeneous nuclear ribonucleoprotein D-like promotes the growth of human colon cancer SW620 cells via its regulation of cell-cycle
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致癌异质核核糖核蛋白D样通过调节细胞周期促进人结肠癌SW620细胞的生长
DOI：
10.1093/abbs/gmy085
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发表时间：
2018-07
影响因子：
3.7
通讯作者：
Haixia Zhou
中科院分区：
文献类型：
--
作者：
Pengshan Zhang;Dehuan Ji;Xiaohui Hu;Hengli Ni;Wenjuan Ma;Xiuyan Zhang;Shibing Liao;Zheng Zeng;Yun Zhao;Haixia Zhou
异质核核糖核蛋白（hnRNP）代表RNA结合蛋白的大家族。异质核核糖核蛋白D样（HNRPDL）是该家族的成员。虽然HNRPDL在少数癌症中的异常表达已经被报道，但HNRPDL在结肠癌患者中是否失调以及该蛋白在这些细胞中起什么作用尚不清楚。在这项研究中，我们发现HNRPDL在结肠癌标本中比对照组显著上调。我们还证明了HNRPDL沉默抑制SW 620细胞在体外和体内的生长。相反，我们构建了一个逆转录病毒载体，将HNRPDL导入非恶性NIH-3 T3细胞，并将这些细胞注射到裸鼠体内。过表达HNRPDL的NIH-3 T3细胞在裸鼠体内产生肿瘤，而对照细胞则没有。机制上，HNRPDL促进细胞周期进程与细胞周期蛋白D3和Ki-67的表达增强，但减少p53和p21的表达。总之，我们的数据表明，HNRPDL在结肠癌细胞中异常表达，其通过激活细胞周期进程促进这些细胞的生长。
Heterogeneous nuclear ribonucleoproteins (hnRNPs) represent a large family of RNA-binding proteins. Heterogeneous nuclear ribonucleoprotein D-like (HNRPDL) is a member of this family. Though aberrant expression of HNRPDL has been reported in a few cancers, whether HNRPDL is deregulated in colon cancer patients and what role this protein plays in these cells are not known yet. In this study, we found that HNRPDL was significantly up-regulated in colon cancer specimens than control. We also demonstrated that HNRPDL silencing inhibited the growth of SW620 cells both in vitro and in vivo. Conversely, we constructed a retroviral vector to deliver HNRPDL into non-malignant NIH-3T3 cells and injected these cells into nude mice. HNRPDL-overexpressing NIH-3T3 cells generated tumors in nude mice but not the control cells. Mechanistically, HNRPDL promoted cell-cycle progression associated with enhanced expressions of cyclin D3 and Ki-67 but decreased expressions of p53 and p21. Taken together, our data demonstrate that HNRPDL is aberrantly expressed in colon cancer cells, which promotes the growth of these cells by activating cell-cycle progression.
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影响因子：
4.5
作者：
Reboll, Marc Rene;Oumard, Andre;Nourbakhsh, Mahtab
通讯作者：
Nourbakhsh, Mahtab
生长停滞特异性 2 在慢性粒细胞白血病细胞中表达上调，并且是其生长所必需的
影响因子：
3.7
作者：
Zhou H;Ge Y;Sun L;Ma W;Wu J;Zhang X;Hu X;Eaves CJ;Wu D;Zhao Y
通讯作者：
Zhao Y
影响因子：
3.1
作者：
Akindahunsi, AA;Bandiera, A;Manzini, G
通讯作者：
Manzini, G
影响因子：
6.4
作者：
Navarro S;Marinelli P;Diaz-Caballero M;Ventura S
通讯作者：
Ventura S
影响因子：
3.3
作者：
de Wit, Meike;Kant, Huub;Fijneman, Remond J. A.
通讯作者：
Fijneman, Remond J. A.