Downregulation of NEDD9 by apigenin suppresses migration, invasion, and metastasis of colorectal cancer cells.
Downregulation of NEDD9 by apigenin suppresses migration, invasion, and metastasis of colorectal cancer cells.
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DOI：
10.1016/j.taap.2016.09.016
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发表时间：
2016-11-15
影响因子：
3.8
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Dai J;Van Wie PG;Fai LY;Kim D;Wang L;Poyil P;Luo J;Zhang Z
芹菜素是一种天然黄酮类化合物，在包括结直肠癌在内的多种癌症中具有抗增殖、抗炎、抗转移等多种抗癌活性。神经前体细胞发育下调蛋白9(NEDD9)是Cas家族的一个多结构域支架蛋白，已被证明与肿瘤的转移和进展有关。本研究探讨NEDD9在芹菜素抑制的大肠腺癌DLD1和SW480细胞迁移、侵袭和转移中的作用。结果表明，NEDD9基因敲除抑制了细胞的迁移、侵袭和转移，过表达NEDD9促进了DLD1和SW4890细胞的迁移和侵袭。芹菜素可在蛋白水平抑制NEDD9的表达，减少FAK、Src和Akt的磷酸化，从而抑制DLD1和SW480细胞的迁移、侵袭和转移。目前的研究表明，芹菜素通过NEDD9/Src/Akt级联途径抑制结直肠癌细胞的迁移、侵袭和转移。NEDD9可作为评估癌症侵袭性和选择抗癌进展的治疗药物的生物标志物。
Apigenin is a natural flavonoid which possesses multiple anti-cancer properties such as anti-proliferation, anti-inflammation, and anti-metastasis in many types of cancers including colorectal cancer. Neural precursor cell expressed developmentally downregulated 9 (NEDD9) is a multi-domain scaffolding protein of the Cas family which has been shown to correlate with cancer metastasis and progression. The present study investigates the role of NEDD9 in apigenin-inhibited cell migration, invasion, and metastasis of colorectal adenocarcinoma DLD1 and SW480 cells. The results show that knockdown of NEDD9 inhibited cell migration, invasion, and metastasis and that overexpression of NEDD9 promoted cell migration and invasion of DLD1 cells and SW4890 cells. Apigenin treatment attenuated NEDD9 expression at protein level, resulting in reduced phosphorylations of FAK, Src, and Akt, leading to inhibition on cell migration, invasion, and metastasis of both DLD1 and SW480 cells. The present study has demonstrated that apigenin inhibits cell migration, invasion, and metastasis through NEDD9/Src/Akt cascade in colorectal cancer cells. NEDD9 may function as a biomarker for evaluation of cancer aggressiveness and for selection of therapeutic drugs against cancer progression.
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影响因子：
8
作者：
Li, Y.;Bavarva, J. H.;Wang, Z.;Guo, J.;Qian, C.;Thibodeau, S. N.;Golemis, E. A.;Liu, W.
通讯作者：
Liu, W.
影响因子：
3.8
作者：
Lee, Wei-Jiunn;Chen, Wen-Kang;Tseng, Tsui-Hwa
通讯作者：
Tseng, Tsui-Hwa
芹菜素通过粘着斑激酶抑制人卵巢癌A2780细胞的迁移和侵袭
影响因子：
4.7
作者：
Hu, Xiao-Wen;Meng, Dan;Fang, Jing
通讯作者：
Fang, Jing
影响因子：
2.9
作者：
Fenton, JI;Hord, NG
通讯作者：
Hord, NG
影响因子：
4.7
作者：
Kaur P;Shukla S;Gupta S
通讯作者：
Gupta S