Activation of innate immunity accelerates sialoadenitis in a mouse model for Sjögren's syndrome-like disease.
Activation of innate immunity accelerates sialoadenitis in a mouse model for Sjögren's syndrome-like disease.
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DOI：
10.1111/j.1601-0825.2011.01839.x
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发表时间：
2011-11
期刊：
影响因子：
3.8
通讯作者：
Deshmukh US
中科院分区：
文献类型：
--
作者：
Nandula SR;Scindia YM;Dey P;Bagavant H;Deshmukh US
干燥综合征是一种慢性自身免疫性疾病，其特征是唾液腺和泪腺内进行性淋巴细胞浸润。本研究旨在研究先天性免疫激活对遗传易感性干燥综合征样疾病发展的小鼠品系的涎腺炎的影响。雌性新西兰黑×新西兰白色F1小鼠反复接受toll样3受体激动剂poly（I：C）处理。通过免疫组化和定量PCR检测不同时间点下颌下腺的涎腺炎和不同趋化因子的基因表达。流式细胞仪检测颌下腺浸润细胞的特征。Poly（I：C）处理显著上调了颌下腺内多种趋化因子的表达。在聚（I：C）处理的小鼠中，涎腺炎的严重程度和发生率显著较高。在最初的炎性细胞浸润中，树突状细胞和NK细胞占优势，其次是CD4+ T细胞。我们的数据清楚地表明，先天免疫的系统激活加速了干燥综合征样疾病小鼠模型的涎腺炎。这些发现表明先天免疫的慢性激活可以影响干燥综合征的某些特征。
Sjögren’s syndrome is a chronic autoimmune disorder characterized by progressive lymphocytic infiltration within the salivary and lacrimal glands. The current study was undertaken to investigate the effects of innate immunity activation on sialoadenitis in a mouse strain genetically susceptible for development of Sjögren’s syndrome-like disease. Female New Zealand Black × New Zealand White F1 mice were repeatedly treated with toll-like 3 receptor agonist poly(I:C). Submandibular glands were investigated at different time points for sialoadenitis by immunohistochemistry and for gene expression of different chemokines by quantitative PCR. Submandibular gland infiltrating cells were characterized by flow cytometry. Poly(I:C) treatment significantly upregulated the expression of multiple chemokines within the submandibular glands. The severity and incidence of sialoadenitis was considerably higher in poly(I:C) treated mice. There was a preponderance of dendritic cells and NK cells in the initial inflammatory cell infiltrates and these were followed by CD4+ T cells. Our data clearly demonstrates that systemic activation of innate immunity accelerates sialoadenitis in a mouse model for Sjögren’s syndrome-like disease. These findings suggest that chronic activation of innate immunity can influence certain features of Sjögren’s syndrome.
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DOI：
10.1111/j.1600-0714.2008.00700.x
发表时间：
2009-01
期刊：
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
影响因子：
--
作者：
Deshmukh US;Nandula SR;Thimmalapura PR;Scindia YM;Bagavant H
通讯作者：
Bagavant H
影响因子：
64.8
作者：
Alexopoulou, L;Holt, AC;Flavell, RA
通讯作者：
Flavell, RA
影响因子：
4.4
作者：
Touil, Tarik;Fitzgerald, Denise;Gran, Bruno
通讯作者：
Gran, Bruno
影响因子：
4.2
作者：
Gottenberg, J. -E.
通讯作者：
Gottenberg, J. -E.
DOI：
10.1016/0090-1229(87)90176-0
发表时间：
1987-01-01
期刊：
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY
影响因子：
--
作者：
JONSSON, R;TARKOWSKI, A;KLARESKOG, L
通讯作者：
KLARESKOG, L