Exploring regulation genes involved in the expression of L-amino acid oxidase in Pseudoalteromonas sp. Rf-1.
Exploring regulation genes involved in the expression of L-amino acid oxidase in Pseudoalteromonas sp. Rf-1.
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DOI：
10.1371/journal.pone.0122741
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Qiu J
中科院分区：
文献类型：
--
作者：
Yu Z;Wang J;Lin J;Zhao M;Qiu J
细菌L-氨基酸氧化酶被认为在海洋生态位中发挥着重要的生物学和生态学作用，因此越来越多地引起人们对其产生调控机制的关注。在本研究中，我们对海洋细菌假交替单胞菌产生LAAO的相关基因进行了研究。转座子诱变RF-1。在筛选的4000多个突变体中，有15个突变体的LAAO活性发生了显著变化。在12个突变体中证实了预期的转座子插入，在这些突变体中发现了中断的基因和相应的功能。对LAAO活性和LAO基因表达的分析表明，GntR家族转录调节因子、甲基酶、非核糖体多肽合成酶、TonB依赖的血红素受体家族、Na+/H+逆向转运体和相关的亚砷酸盐渗透酶、N-乙酰转移酶GCN5、酮酸还原异构酶和SAM依赖的甲基转移酶，及其编码基因可能在转录、转录后、翻译和/或翻译后水平上参与上调或下调途径。将nhad和sdmt基因分别互补到相应的突变体中，使其失去LAAO活性。任何一个基因的互补都可以恢复LAAO的活性和LAO基因的表达，证明了它们在LAAO生物合成中的调节作用。本研究首次对互隔假单胞菌LAAO产生的分子调控机制进行了研究。Rf-1，这对于更好地了解LAAO的生物学和生态学作用是重要的。
Bacterial L-amino acid oxidase (LAAO) is believed to play important biological and ecological roles in marine niches, thus attracting increasing attention to understand the regulation mechanisms underlying its production. In this study, we investigated genes involved in LAAO production in marine bacterium Pseudoalteromonas sp. Rf-1 using transposon mutagenesis. Of more than 4,000 mutants screened, 15 mutants showed significant changes in LAAO activity. Desired transposon insertion was confirmed in 12 mutants, in which disrupted genes and corresponding functionswere identified. Analysis of LAAO activity and lao gene expression revealed that GntR family transcriptional regulator, methylase, non-ribosomal peptide synthetase, TonB-dependent heme-receptor family, Na+/H+ antiporter and related arsenite permease, N-acetyltransferase GCN5, Ketol-acid reductoisomerase and SAM-dependent methytransferase, and their coding genes may be involved in either upregulation or downregulation pathway at transcriptional, posttranscriptional, translational and/or posttranslational level. The nhaD and sdmT genes were separately complemented into the corresponding mutants with abolished LAAO-activity. The complementation of either gene can restore LAAO activity and lao gene expression, demonstrating their regulatory role in LAAO biosynthesis. This study provides, for the first time, insights into the molecular mechanisms regulating LAAO production in Pseudoalteromonas sp. Rf-1, which is important to better understand biological and ecological roles of LAAO.
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影响因子：
3.7
作者：
Okubo BM;Silva ON;Migliolo L;Gomes DG;Porto WF;Batista CL;Ramos CS;Holanda HH;Dias SC;Franco OL;Moreno SE
通讯作者：
Moreno SE
影响因子：
11.4
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Lee ML;Fung SY;Chung I;Pailoor J;Cheah SH;Tan NH
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11.4
作者：
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通讯作者：
Varshney, Umesh
影响因子：
3.2
作者：
HERRERO, M;DELORENZO, V;TIMMIS, KN
通讯作者：
TIMMIS, KN