Less is more: unveiling the functional core of hematopoietic stem cells through knockout mice.
Less is more: unveiling the functional core of hematopoietic stem cells through knockout mice.
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DOI：
10.1016/j.stem.2012.08.006
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发表时间：
2012-09-07
期刊：
影响因子：
23.9
通讯作者：
Goodell, Margaret A.
中科院分区：
文献类型：
--
作者：
Rossi, Lara;Lin, Kuanyin K.;Boles, Nathan C.;Yang, Liubin;King, Katherine Y.;Jeong, Mira;Mayle, Allison;Goodell, Margaret A.
造血干细胞（HSC）是最早被认可的成体干细胞之一。因此，已经在敲除小鼠中检查了近 200 个基因在 HSC 功能中的作用。在这篇综述中，我们编译了大部分报告，以对这些遗传分析揭示的功能模块进行广泛的概述，并重点介绍一些涉及的关键调控途径，包括细胞周期控制、TGF-β信号传导、Pten/AKT信号传导、Wnt信号传导和细胞因子信号传导。最后，我们提出了基因敲除小鼠 HSC 功能表征的建议，以促进交叉研究比较，从而生成更有凝聚力的 HSC 生物学图景。在设计领域，极简主义运动将建筑物和物体剥离到最基本的特征，建筑师路德维希·密斯·凡德罗在他的座右铭“少即是多”中总结了这一观点。通过耗尽特定基因的 HSC，敲除研究将极简主义方法转入研究生物学，提供对功能良好的造血系统不可或缺的遗传特征的基本核心的见解。
Hematopoietic stem cells (HSCs) represent one of the first recognized somatic stem cells. As such, nearly 200 genes have been examined for roles in HSC function in knockout mice. In this review, we compile the majority of these reports to provide a broad overview of the functional modules revealed by these genetic analyses and highlight some key regulatory pathways involved, including cell cycle control, TGF-β signaling, Pten/AKT signaling, Wnt signaling, and cytokine signaling. Finally, we propose recommendations for characterization of HSC function in knockout mice to facilitate cross-study comparisons that would generate a more cohesive picture of HSC biology. In the field of design, the minimalist movement stripped down buildings and objects to their most basic features, a sentiment that architect Ludwig Mies van der Rohe summarized in his motto “less is more”. By depleting HSCs of specific genes, knockout studies transpose the minimalist approach into research biology, providing insights into the essential core of genetic features that is indispensable for a well-functioning hematopoietic system.
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