Optimization of the Fermentation Media and Parameters for the Bio-control Potential of Trichoderma longibrachiatum T6 Against Nematodes.
Optimization of the Fermentation Media and Parameters for the Bio-control Potential of Trichoderma longibrachiatum T6 Against Nematodes.
复制标题
DOI：
10.3389/fmicb.2020.574601
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Xu B
中科院分区：
文献类型：
--
作者：
Zhang S;Gan Y;Liu J;Zhou J;Xu B
谷物囊线虫是谷物作物的重要土传病原体之一，在世界范围内造成严重的产量损失。木霉制剂作为商业生物防治剂用于防治土壤传播的植物病原体，如H. avenae。然而，长尾木霉发酵参数与其对avenae的生物防治潜力之间的关系尚未完全确定。本研究采用单因素法和Plackett-Burman设计评价了10种不同发酵培养基和条件对长链achiatum T6 （T. longibrachiatum T6）杀线虫活性的影响，并采用Box-Behnken设计考察了不同发酵参数之间的相互作用。采用响应面法（RSM）对提高T6培养滤液杀线虫活性的变量进行了探索和优化。小培养基（Minor Medium， MM）加麦麸-2培养基是T6培养滤液对黄颡鱼（H. avenae）第二阶段幼虫（J2s）最有效的发酵培养基。T6培养滤液在初始pH 6、28℃培养温度、180转/分转速、发酵培养基60 ml、培养时间7 d、接种量1 ml的条件下，获得了J2s的最大死亡率。其中，初始pH、接种量和孵育天数是提高T6培养滤液杀线虫活性的最显著参数和关键自变量。在统计预测的基础上进一步优化后，T6培养滤液在初始pH为6.06、接种量为1.62 ml、培养时间为7.15 d的条件下发酵，杀线虫活性最高，达到92.42%。与优化前相比，其杀线虫活性提高了约1.07%。在温室试验中，T6培养滤液在小麦播种后第70天的生物防治效果为83.88%。验证实验结果与模型预测一致。本研究提高了木霉对植物寄生线虫的生物防治潜力，为未来新型生物防治剂的开发提供了经济高效的生物资源。
The cereal cyst nematode Heterodera avenae is one of the important soil-borne pathogens of cereal crops and causes high yield losses worldwide. Trichoderma spp. formulations are applied as commercial bio-control agents against soil-borne plant pathogens such as H. avenae. However, the relationship between Trichoderma longibrachiatum fermentation parameters and its bio-control potential against H. avenae has not been exclusively established. In the present study, the effect of 10 different fermentation media and conditions on the nematicidal activity of T. longibrachiatum T6 (T6) was evaluated with a single-factor method and a Plackett–Burman design, and the interaction between different fermentation parameters was investigated by a Box–Behnken design. The variables for enhancing the nematicidal activity of T6 culture filtrates were explored and optimized using response surface methodology (RSM). The Minor Medium (MM) plus wheat bran-2 medium was found to be the most effective fermentation medium for T6 culture filtrates against the second stage juveniles (J2s) of H. avenae. The maximum mortality of the J2s was obtained using the T6 culture filtrates under the following fermentation conditions: initial pH 6, 28°C culture temperature, 180 rpm rotating speed, 60 ml of fermentation media, 7 days of incubation time, and 1 ml of inoculation volumes. Among these parameters, the initial pH, inoculation volume, and incubation day were identified as the most significant parameters and critical independent variables for enhancing the nematicidal activity of the T6 culture filtrates. After further optimizations based on statistical predictions, the highest nematicidal activity (92.42%) was obtained with the T6 culture filtrates fermented under an initial pH of 6.06, an inoculation volume of 1.62 ml, and an incubation time of 7.15 days. The nematicidal activity was increased approximately by as high as 1.07% compared with that before optimization. Bio-control efficacy of T6 culture filtrates was 83.88% at the 70th day after wheat seeds sowing in greenhouse experiments. The results from the validation experiments agreed with the model predictions. Our study has improved the bio-control potential of Trichoderma spp. against the plant-parasitic nematodes H. avenae and provided a cost-efficient bio-resource in the future development of novel bio-control agents.
登录
查看更多内容
影响因子：
1.8
作者：
Larentis AL;Nicolau JF;Esteves Gdos S;Vareschini DT;de Almeida FV;dos Reis MG;Galler R;Medeiros MA
通讯作者：
Medeiros MA
影响因子：
1.8
作者：
Long, Haibo;Peng, Huan;Peng, Deliang
通讯作者：
Peng, Deliang
影响因子：
3.8
作者：
Sharma, Abhishek;Sharma, Satyawati;Dalela, Manu
通讯作者：
Dalela, Manu
影响因子：
3.2
作者：
Sharon, E;Bar-Eyal, M;Spiegel, Y
通讯作者：
Spiegel, Y
影响因子：
4.1
作者：
Ambati, P;Ayyanna, C
通讯作者：
Ayyanna, C