Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV.
Inactivation of the coronavirus that induces severe acute respiratory syndrome, SARS-CoV.
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DOI:
10.1016/j.jviromet.2004.06.006
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发表时间:
2004-10
影响因子:
3.1
通讯作者:
Taylor DR
中科院分区:
文献类型:
--
作者:
Darnell ME;Subbarao K;Feinstone SM;Taylor DR
Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 °C or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels.
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