Cloning and sequencing of a cDNA encoding the Rieske iron-sulfur protein of bovine heart mitochondrial ubiquinol-cytochrome c reductase.

Cloning and sequencing of a cDNA encoding the Rieske iron-sulfur protein of bovine heart mitochondrial ubiquinol-cytochrome c reductase.
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编码牛心脏线粒体泛醇-细胞色素 c 还原酶 Rieske 铁硫蛋白的 cDNA 的克隆和测序。

DOI:
10.1016/0006-291x(90)92063-6
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发表时间:
1990
影响因子:
3.1
通讯作者:
Yu,CA
Yu,CA
中科院分区:
生物学4区
文献类型:
--
作者:
Usui,S;Yu,L;Yu,CA

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Two cDNA clones encoding bovine heart mitochondrial Rieske iron-sulfur protein were obtained by immunological screening of a bovine heart cDNA expression library in λgt11 with antiserum directed against Rieske iron-sulfur protein isolated from bovine heart mitochondrial ubiquinol-cytochromecreductase. The cDNA inserts were 1005 and 1100 base pairs with an open reading frame of 807 base pairs which encoded a 196-amino acid mature Rieske iron-sulfur protein and a 73-amino acid presequence. The amino acid sequence of Rieske iron-sulfur protein deduced from nucleotide sequencing is the same as that obtained from protein sequencing except at residues #73 and #191 which are Ser and Asp instead of Ala and Gly, respectively.
Lambda gt 11:使用抗体探针进行基因分离和其他应用。
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DOI: --
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DOI: 10.1021/bi00566a008
发表时间: 1980
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作者:
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通讯作者: Yu,L