Expression of the cell surface-associated glycoprotein, fibronectin, in the early mouse embryo.
Expression of the cell surface-associated glycoprotein, fibronectin, in the early mouse embryo.
复制标题
细胞表面相关糖蛋白纤连蛋白在早期小鼠胚胎中的表达。
DOI：
10.1016/0012-1606(79)90289-6
复制
发表时间：
1979
影响因子：
2.7
通讯作者：
A. Vaheri
中科院分区：
文献类型：
--
作者：
J. Wartiovaara;I. Leivo;A. Vaheri
通过间接免疫荧光法研究小鼠胚胎发生早期细胞表面相关糖蛋白纤连蛋白的表达。在早期植入前胚胎中未检测到纤连蛋白。微量的蛋白质首先在晚期囊胚的内细胞团的细胞之间发现。在植入的早期卵缸中，纤连蛋白沉积在内细胞团的外胚层和内胚层之间以及新生的赖克特膜中。随着发育，内脏和壁层内胚层细胞对该蛋白呈阳性，但在外胚层细胞中未检测到纤连蛋白。在中胚层与外胚层分离期间，纤连蛋白出现在中胚层细胞中并作为两个胚层之间的带。在发育中的羊膜和绒毛膜中，该蛋白质位于外胚层和中胚层细胞层之间。结果表明，纤连蛋白是小鼠囊胚内细胞团内胚层形成阶段的早期分化市场。它也是中胚层外观的标志，似乎与发育中胚胎中积累的细胞外基质材料有关。
The expression of the cell surface-associated glycoprotein fibronectin was studied by indirect immunofluorescence in the early stages of mouse embryogenesis. Fibronectin was not detectable in early preimplantation embryos. Trace amounts of the protein were first found between the cells of the inner cell mass of late blastocysts. In implanted early egg cylinders, fibronectin was deposited between the ectoderm and endoderm of the inner cell mass and in the nascent Reichert's membrane. With development, the visceral and the parietal endoderm cells became positive for the protein, but no fibronectin was detected in ectoderm cells. During segregation of mesoderm from ectoderm, fibronectin appeared in mesoderm cells and as a band between the two germ layers. In the developing amnion and chorion, the protein was localized between the ectodermal and mesodermal cell layers. The results indicate that fibronectin is an early differentiation market for the stage of endoderm formation in the inner cell mass of the mouse blastocyst. It is also a marker of mesoderm appearance and seems to be associated with the accumulating extracellular matrix material in the developing embryo.
DOI：
--
发表时间：
1978-02
期刊：
Journal of embryology and experimental morphology
影响因子：
--
作者：
By M. Dziadek;E. Adamson
通讯作者：
By M. Dziadek;E. Adamson