Production of interleukin-12 and expression of its receptors by murine microglia
Production of interleukin-12 and expression of its receptors by murine microglia
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小鼠小胶质细胞白细胞介素 12 的产生及其受体的表达
DOI：
10.1016/s0006-8993(97)01166-9
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发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
T. Takayanagi
中科院分区：
文献类型：
--
作者：
A. Suzumura;M. Sawada;T. Takayanagi
采用ELISA检测异二聚体p70， RT-PCR检测编码p35和p40的mRNA表达，检测培养小鼠小胶质细胞和星形胶质细胞产生白细胞介素-12 （IL-12）。小胶质细胞，而不是星形胶质细胞，在脂多糖和干扰素-γ的作用下产生il - 12p70。在上述刺激下，小胶质细胞系Ra2只产生p40，而不产生p35。因此，一些小胶质细胞群可能通过在中枢神经系统中产生IL-12来诱导辅助性1型T细胞反应。诱导小胶质细胞表达编码IL-12受体的mRNA，该受体仅在活化的T细胞和NK细胞中表达。
Production of interleukin-12 (IL-12) by cultured murine microglia and astrocytes was examined, by means of ELISA to detect heterodimeric p70 and RT-PCR to analyze the expression of mRNA encoding p35 and p40. Microglia, but not astrocytes, produced IL-12 p70 in response to lipopolysaccharide and interferon-γ. The microglial cell line, Ra2, produced only p40, but not p35, upon above stimulation. Thus, it is possible that some population of microglia induce helper 1 type T cell response via producing IL-12 in the CNS. Microglia were induced to express mRNA encoding IL-12 receptors which were exclusively expressed in activated T and NK cells.
影响因子：
4.4
作者：
K. Selmaj;M. Farooq;W. T. Norton;C. Raine;C. Brosnan
通讯作者：
K. Selmaj;M. Farooq;W. T. Norton;C. Raine;C. Brosnan