Brain-Derived Neurotrophic Factor Enhances Long-Term Potentiation in Rat Visual Cortex
Brain-Derived Neurotrophic Factor Enhances Long-Term Potentiation in Rat Visual Cortex
复制标题
脑源性神经营养因子增强大鼠视觉皮层的长时程增强
DOI：
10.1523/jneurosci.17-17-06707.1997
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
H. Hatanaka
中科院分区：
文献类型：
--
作者：
Y. Akaneya;T. Tsumoto;S. Kinoshita;H. Hatanaka
脑源性神经营养因子 (BDNF) 和神经营养蛋白-3 (NT-3) 是神经生长因子 (NGF) 基因家族的成员，已被认为在发育中的神经系统中神经网络的经验依赖性修饰中发挥作用。在这项研究中，我们解决了这些神经营养素是否参与视觉皮层发育中的长时程增强（LTP）的问题。我们记录了在幼鼠（出生后第 15-25 天）制备的视觉皮层切片中以 0.1 Hz 测试刺激第 IV 层引起的第 II/III 层场电位和全细胞电流，并观察 BDNF、NT-3 和 NGF 对这些反应的影响。然后我们分析了这些神经营养因子对第四层强直（θ-爆发型）刺激诱导的 LTP 的影响。我们发现，在大多数情况下，200 ng/ml 的 BDNF 会增强场电位和 EPSC，并且这种增强作用在 BDNF 应用停止后持续存在。在20ng/ml的浓度下，BDNF没有表现出这样的效果，但它增强了LTP表达的幅度。另一方面，NT-3 和 NGF 则没有这些作用。用抗 BDNF 抗体对切片进行免疫组织化学染色显示，外源 BDNF 在其应用后约 5 分钟内渗透到整个切片中。通过将切片与 TrkB-IgG 融合蛋白（一种 BDNF 清除剂）预孵育，或同时应用 K252a（一种受体酪氨酸激酶抑制剂），可以阻断 BDNF 的作用。 TrkB-IgG 或 K252a 本身完全阻断 LTP，表明内源性 BDNF 或另一种 TrkB 配体在发育中的视觉皮层的 LTP 中发挥作用。
Brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), members of the nerve growth factor (NGF) gene family, have been suggested to play a role in experience-dependent modification of neural networks in the developing nervous system. In this study we addressed the question of whether these neurotrophins are involved in long-term potentiation (LTP) in developing visual cortex. We recorded layer II/III field potentials and whole-cell currents evoked by test stimulation of layer IV at 0.1 Hz in visual cortical slices prepared from young rats (postnatal day 15–25) and observed effects of BDNF, NT-3, and NGF on these responses. Then we analyzed the effects of these neurotrophins on LTP induced by tetanic (Θ-burst type) stimulation of layer IV. We found that BDNF at 200 ng/ml potentiated field potentials and EPSCs in most cases and that this potentiation lasted after cessation of the BDNF application. At the concentration of 20 ng/ml, BDNF did not show such an effect, but it enhanced the magnitude of expressed LTP. On the other hand, NT-3 and NGF had none of these effects. Immunohistochemical staining of slices with antibody against BDNF showed that exogenous BDNF penetrated into the whole slice within ∼5 min of its application. The actions of BDNF were blocked by preincubation of slices with TrkB-IgG fusion protein, a BDNF scavenger, or coapplication of K252a, an inhibitor for receptor tyrosine kinases. TrkB-IgG or K252a itself completely blocked LTP, suggesting that endogenous BDNF or another TrkB ligand plays a role in LTP in the developing visual cortex.
影响因子：
4.6
作者：
R. Klein;D. Martín-Zanca;M. Barbacid;L. Parada
通讯作者：
R. Klein;D. Martín-Zanca;M. Barbacid;L. Parada
DOI：
10.1073/pnas.92.17.8074
发表时间：
1995-08-15
影响因子：
11.1
作者：
LEVINE, ES;DREYFUS, CF;PLUMMER, MR
通讯作者：
PLUMMER, MR