Conditional cell reprogramming for modeling host-virus interactions and human viral diseases.
Conditional cell reprogramming for modeling host-virus interactions and human viral diseases.
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DOI：
10.1002/jmv.26093
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发表时间：
2020-11
影响因子：
12.7
通讯作者：
Mondal AM
中科院分区：
文献类型：
--
作者：
Liu X;Mondal AM
常规癌症和转化细胞系广泛用于癌症生物学和生物学中的其它领域。这些细胞通常具有来自原始肿瘤本身的异常，但也可能由于遗传操作或长期传代期间的遗传和表观遗传变化而发生异常。原代培养物可以保持原始组织类型的谱系功能，但由于细胞衰老的性质，它们具有非常有限的寿命或群体倍增时间。原代培养物的产量通常很低，并且与任何原始组织标本的差异很大，这在很大程度上限制了它们在研究中的应用。动物模型通常用于病毒感染的研究、疾病建模、抗病毒药物和疫苗的开发。由于细胞表面上的可变受体，人类病毒通常需要在体内进行一系列传代以适应宿主环境，并且可能具有来自细胞类型或宿主物种的细胞内限制。在这里，我们描述了一个长期的细胞培养系统，条件性重编程细胞（CRC），及其在人类病毒性疾病和药物发现建模中的应用。在Y-27632（条件性重编程，CR）存在的情况下使用饲养层共培养，可以从手术标本、芯或针活检以及其他微创或非侵入性标本（例如鼻腔刷拭）中获得并快速繁殖CRC。CRCs保留了其谱系功能，并提供生物学相关和生理条件，适用于研究病毒进入和复制，宿主细胞的先天免疫应答以及发现抗病毒药物。在这篇综述中，我们总结了CR技术在模拟宿主-病毒相互作用和人类病毒性疾病（包括严重急性呼吸综合征冠状病毒-2和冠状病毒疾病-2019）以及抗病毒发现中的应用。
Conventional cancer and transformed cell lines are widely used in cancer biology and other fields within biology. These cells usually have abnormalities from the original tumor itself, but may also develop abnormalities due to genetic manipulation, or genetic and epigenetic changes during long‐term passages. Primary cultures may maintain lineage functions as the original tissue types, yet they have a very limited life span or population doubling time because of the nature of cellular senescence. Primary cultures usually have very low yields, and the high variability from any original tissue specimens, largely limiting their applications in research. Animal models are often used for studies of virus infections, disease modeling, development of antiviral drugs, and vaccines. Human viruses often need a series of passages in vivo to adapt to the host environment because of variable receptors on the cell surface and may have intracellular restrictions from the cell types or host species. Here, we describe a long‐term cell culture system, conditionally reprogrammed cells (CRCs), and its applications in modeling human viral diseases and drug discovery. Using feeder layer coculture in presence of Y‐27632 (conditional reprogramming, CR), CRCs can be obtained and rapidly propagated from surgical specimens, core or needle biopsies, and other minimally invasive or noninvasive specimens, for example, nasal cavity brushing. CRCs preserve their lineage functions and provide biologically relevant and physiological conditions, which are suitable for studies of viral entry and replication, innate immune responses of host cells, and discovery of antiviral drugs. In this review, we summarize the applications of CR technology in modeling host‐virus interactions and human viral diseases including severe acute respiratory syndrome coronavirus‐2 and coronavirus disease‐2019, and antiviral discovery.
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影响因子：
3.7
作者：
Armstrong, Andrew J.;Gupta, Santosh;George, Daniel J.
通讯作者：
George, Daniel J.
影响因子：
3.7
作者：
Gardell AM;Qin Q;Rice RH;Li J;Kültz D
通讯作者：
Kültz D
DOI：
10.1002/pros.23206
发表时间：
2016-09
期刊：
The Prostate
影响因子：
--
作者：
Ellis L;Ku S;Li Q;Azabdaftari G;Seliski J;Olson B;Netherby CS;Tang DG;Abrams SI;Goodrich DW;Pili R
通讯作者：
Pili R
DOI：
10.1126/science.1254721
发表时间：
2014-12-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Crystal AS;Shaw AT;Sequist LV;Friboulet L;Niederst MJ;Lockerman EL;Frias RL;Gainor JF;Amzallag A;Greninger P;Lee D;Kalsy A;Gomez-Caraballo M;Elamine L;Howe E;Hur W;Lifshits E;Robinson HE;Katayama R;Faber AC;Awad MM;Ramaswamy S;Mino-Kenudson M;Iafrate AJ;Benes CH;Engelman JA
通讯作者：
Engelman JA
DOI：
10.1165/rcmb.2016-0276ma
发表时间：
2017-05-01
影响因子：
6.4
作者：
Gentzsch, Martina;Boyles, Susan E.;Randell, Scott H.
通讯作者：
Randell, Scott H.