Somatic Mutation Analysis in Salix suchowensis Reveals Early-Segregated Cell Lineages.
Somatic Mutation Analysis in Salix suchowensis Reveals Early-Segregated Cell Lineages.
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Salix Suchowensis 的体细胞突变分析揭示了早期分离的细胞谱系
DOI：
10.1093/molbev/msab286
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发表时间：
2021-12-09
影响因子：
10.7
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Ren Y;He Z;Liu P;Traw B;Sun S;Tian D;Yang S;Jia Y;Wang L
长寿植物在其漫长的生命周期中面临着不断增加的突变负担的挑战。分生干细胞的早期隔离被认为可以有效地减缓这一过程，但是直接测量植物中伴随分离细胞系的体细胞突变仍然很少见。在这里，我们追踪了灌木柳树（Salix suchowensis） 8个主要分支上22个茎插枝的33个叶片和22个不定根的体细胞突变。我们发现大多数突变在叶和根中分别繁殖，为潜在细胞系的早期分离提供了明确的证据。通过结合谱系追踪和等位基因频率分析，我们的结果揭示了一系列不同分支共有的突变，但只存在于叶片而不存在于根中。这些突变可能是通过快速分裂的体细胞谱系传播的，这些体细胞谱系可以在分支的多次迭代中存活下来，与缓慢分裂的腋窝干细胞谱系不同。因此，叶片是由缓慢和快速分裂的细胞系贡献的，导致繁殖突变的固定机会不同。相比之下，每个根可能来自于不定干细胞谱系中的单个创始细胞。我们的研究结果提供了直接的证据，表明分生组织的早期分离减缓了腋生分生组织的突变积累，这意味着通过趋同进化，植物的“种系”与动物的种系类似。
Long-lived plants face the challenge of ever-increasing mutational burden across their long lifespan. Early sequestration of meristematic stem cells is supposed to efficiently slow down this process, but direct measurement of somatic mutations that accompanies segregated cell lineages in plants is still rare. Here, we tracked somatic mutations in 33 leaves and 22 adventitious roots from 22 stem-cuttings across eight major branches of a shrub willow (Salix suchowensis). We found that most mutations propagated separately in leaves and roots, providing clear evidence for early segregation of underlying cell lineages. By combining lineage tracking with allele frequency analysis, our results revealed a set of mutations shared by distinct branches, but were exclusively present in leaves and not in roots. These mutations were likely propagated by rapidly dividing somatic cell lineages which survive several iterations of branching, distinct from the slowly dividing axillary stem cell lineages. Leaf is thus contributed by both slowly and rapidly dividing cell lineages, leading to varied fixation chances of propagated mutations. By contrast, each root likely arises from a single founder cell within the adventitious stem cell lineages. Our findings give straightforward evidence that early segregation of meristems slows down mutation accumulation in axillary meristems, implying a plant “germline” paralog to the germline of animals through convergent evolution.
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影响因子：
4.4
作者：
Diwan D;Komazaki S;Suzuki M;Nemoto N;Aita T;Satake A;Nishigaki K
通讯作者：
Nishigaki K
影响因子：
9.2
作者：
Burian, Agata;de Reuille, Pierre Barbier;Kuhlemeier, Cris
通讯作者：
Kuhlemeier, Cris
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
14.9
作者：
De Baets G;Van Durme J;Reumers J;Maurer-Stroh S;Vanhee P;Dopazo J;Schymkowitz J;Rousseau F
通讯作者：
Rousseau F
影响因子：
3
作者：
Carlson, MC
通讯作者：
Carlson, MC