Systematic genome sequence differences among leaf cells within individual trees.
Systematic genome sequence differences among leaf cells within individual trees.
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DOI：
10.1186/1471-2164-15-142
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发表时间：
2014-02-19
期刊：
影响因子：
4.4
通讯作者：
Nishigaki K
中科院分区：
文献类型：
--
作者：
Diwan D;Komazaki S;Suzuki M;Nemoto N;Aita T;Satake A;Nishigaki K
即使在下一代测序（NGS）时代，也不清楚单个生物体中的细胞是否具有系统独特的基因组。解决这个问题，与DNA突变率相关的最基本的生物学问题之一，可以帮助阐明癌症的基本机制。使用基因组图谱分析（GP），我们发现相当大的系统变异的基因组序列细胞在个别木本植物。基因组序列差异的程度（基因组距离）从植物的底部到顶部系统地变化，使得在来自最高分支的叶基因组和树的其余部分之间观察到最大的分歧。在吉野樱桃树和日本山毛榉树中均观察到这种系统变异。如通过GP测量的，个体生物体内两个细胞之间的基因组距离是不可忽略的，并且与物理距离（即，分支到分支距离）。这种现象被认为是每次细胞分裂中突变积累的结果，这意味着分歧的程度与分离两个细胞的代数成正比。
Even in the age of next-generation sequencing (NGS), it has been unclear whether or not cells within a single organism have systematically distinctive genomes. Resolving this question, one of the most basic biological problems associated with DNA mutation rates, can assist efforts to elucidate essential mechanisms of cancer. Using genome profiling (GP), we detected considerable systematic variation in genome sequences among cells in individual woody plants. The degree of genome sequence difference (genomic distance) varied systematically from the bottom to the top of the plant, such that the greatest divergence was observed between leaf genomes from uppermost branches and the remainder of the tree. This systematic variation was observed within both Yoshino cherry and Japanese beech trees. As measured by GP, the genomic distance between two cells within an individual organism was non-negligible, and was correlated with physical distance (i.e., branch-to-branch distance). This phenomenon was assumed to be the result of accumulation of mutations from each cell division, implying that the degree of divergence is proportional to the number of generations separating the two cells.
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DOI：
10.1126/science.1224344
发表时间：
2012-10-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Meyer M;Kircher M;Gansauge MT;Li H;Racimo F;Mallick S;Schraiber JG;Jay F;Prüfer K;de Filippo C;Sudmant PH;Alkan C;Fu Q;Do R;Rohland N;Tandon A;Siebauer M;Green RE;Bryc K;Briggs AW;Stenzel U;Dabney J;Shendure J;Kitzman J;Hammer MF;Shunkov MV;Derevianko AP;Patterson N;Andrés AM;Eichler EE;Slatkin M;Reich D;Kelso J;Pääbo S
通讯作者：
Pääbo S
影响因子：
48
作者：
Flusberg, Benjamin A.;Webster, Dale R.;Lee, Jessica H.;Travers, Kevin J.;Olivares, Eric C.;Clark, Tyson A.;Korlach, Jonas;Turner, Stephen W.
通讯作者：
Turner, Stephen W.
影响因子：
64.8
作者：
Navin N;Kendall J;Troge J;Andrews P;Rodgers L;McIndoo J;Cook K;Stepansky A;Levy D;Esposito D;Muthuswamy L;Krasnitz A;McCombie WR;Hicks J;Wigler M
通讯作者：
Wigler M
影响因子：
56.9
作者：
McVean, GAT;Myers, SR;Donnelly, P
通讯作者：
Donnelly, P
影响因子：
2.7
作者：
NISHIGAKI, K;TSUBOTA, M;HUSIMI, Y
通讯作者：
HUSIMI, Y