Attenuation of cardiac hypertrophy by G-CSF is associated with enhanced migration of bone marrow-derived cells.
Attenuation of cardiac hypertrophy by G-CSF is associated with enhanced migration of bone marrow-derived cells.
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DOI：
10.1111/jcmm.12494
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发表时间：
2015-05
影响因子：
5.3
通讯作者：
Brunner S
中科院分区：
文献类型：
--
作者：
Huber BC;Beetz NL;Laskowski A;Ziegler T;Grabmaier U;Kupatt C;Herbach N;Wanke R;Franz WM;Massberg S;Brunner S
粒细胞集落刺激因子（G-CSF）已被证明可促进骨髓源性干细胞（BMC）动员进入血流，与心肌梗死后生存率和心功能改善相关。因此，本研究的目的是研究G-CSF是否能够减弱压力诱导的LV肥大小鼠模型中的心脏重塑，重点是BMC的动员和迁移。在C57 BL/6 J小鼠中通过横向主动脉缩窄（TAC）诱导LV肥大。TAC术后4周。用G-CSF（100 μg/kg/天; Amgen Biologicals）处理小鼠2周。通过流式细胞术分析心脏中迁移的BMCs的数量。采用RT-PCR和ELISA方法检测心肌组织中不同生长因子的mRNA表达和蛋白水平。在TAC手术后8周通过超声心动图和免疫组织化学分析进行功能分析。G-CSF处理的动物显示VLA-4+和c-kit+ BMCs的归巢增强，与肥大心肌中相应归巢因子血管细胞粘附蛋白1和干细胞因子的mRNA表达和蛋白水平增加相关。在功能上，G-CSF在TAC手术后显著保留了LV功能，与对照动物相比，这与纤维化面积显著减少相关。此外，G-CSF治疗的动物显示出TAC手术后存活率的显著改善。总之，G-CSF治疗保留了心脏功能，并能够减少诱导LV肥大后的心脏纤维化，与VLA-4+和c-kit+ BMC归巢增加及其各自归巢因子VCAM-1和SCF表达增强相关。
Granulocyte-colony stimulating factor (G-CSF) has been shown to promote mobilization of bone marrow-derived stem cells (BMCs) into the bloodstream associated with improved survival and cardiac function after myocardial infarction. Therefore, the aim of the present study was to investigate whether G-CSF is able to attenuate cardiac remodelling in a mouse model of pressure-induced LV hypertrophy focusing on mobilization and migration of BMCs. LV hypertrophy was induced by transverse aortic constriction (TAC) in C57BL/6J mice. Four weeks after TAC procedure. Mice were treated with G-CSF (100 μg/kg/day; Amgen Biologicals) for 2 weeks. The number of migrated BMCs in the heart was analysed by flow cytometry. mRNA expression and protein level of different growth factors in the myocardium were investigated by RT-PCR and ELISA. Functional analyses assessed by echocardiography and immunohistochemical analysis were performed 8 weeks after TAC procedure. G-CSF-treated animals revealed enhanced homing of VLA-4+ and c-kit+ BMCs associated with increased mRNA expression and protein level of the corresponding homing factors Vascular cell adhesion protein 1 and Stem cell factor in the hypertrophic myocardium. Functionally, G-CSF significantly preserved LV function after TAC procedure, which was associated with a significantly reduced area of fibrosis compared to control animals. Furthermore, G-CSF-treated animals revealed a significant improvement of survival after TAC procedure. In summary, G-CSF treatment preserves cardiac function and is able to diminish cardiac fibrosis after induction of LV hypertrophy associated with increased homing of VLA-4+ and c-kit+ BMCs and enhanced expression of their respective homing factors VCAM-1 and SCF.
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影响因子：
5.3
作者：
Brunner S;Huber BC;Weinberger T;Vallaster M;Wollenweber T;Gerbitz A;Hacker M;Franz WM
通讯作者：
Franz WM
骨髓干细胞移植到缺血心肌后的体内功能和转录谱。
DOI：
10.1161/atvbaha.111.238618
发表时间：
2012-01
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Sheikh AY;Huber BC;Narsinh KH;Spin JM;van der Bogt K;de Almeida PE;Ransohoff KJ;Kraft DL;Fajardo G;Ardigo D;Ransohoff J;Bernstein D;Fischbein MP;Robbins RC;Wu JC
通讯作者：
Wu JC
影响因子：
5.2
作者：
Huber, Bruno C.;Ransohoff, Julia D.;Ransohoff, Katherine J.;Riegler, Johannes;Ebert, Antje;Kodo, Kazuki;Gong, Yongquan;Sanchez-Freire, Veronica;Dey, Devaveena;Kooreman, Nigel G.;Diecke, Sebastian;Zhang, Wendy Y.;Odegaard, Justin;Hu, Shijun;Gold, Joseph D.;Robbins, Robert C.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
--
作者：
Baldo, Marcelo P.;Davel, Ana P. C.;Mill, Jose G.
通讯作者：
Mill, Jose G.
影响因子：
82.9
作者：
Kocher, AA;Schuster, MD;Itescu, S
通讯作者：
Itescu, S