Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies.
Virological and immunological features of SARS-CoV-2-infected children who develop neutralizing antibodies.
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DOI：
10.1016/j.celrep.2021.108852
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发表时间：
2021-03-16
期刊：
影响因子：
8.8
通讯作者：
Palma P
中科院分区：
文献类型：
--
作者：
Cotugno N;Ruggiero A;Bonfante F;Petrara MR;Zicari S;Pascucci GR;Zangari P;De Ioris MA;Santilli V;Manno EC;Amodio D;Bortolami A;Pagliari M;Concato C;Linardos G;Campana A;Donà D;Giaquinto C;CACTUS Study Team;Brodin P;Rossi P;De Rossi A;Palma P
随着全球COVID-19大流行的发展，获得儿童对SARS-CoV-2的适应性免疫的知识，以确定免疫或感染后保护的免疫相关性至关重要。我们分析了66名COVID-19感染儿童在症状出现后7（±2）天的抗SARS-CoV-2抗体及其中和活性（PRNT）。具有特异性体液应答的个体表现出更快的病毒清除和更低的病毒载量，并与体外感染性降低相关。我们证明SARS-CoV-2特异性CD 4 + CD 40 L + T细胞和刺突特异性B细胞的频率与抗SARS-CoV-2抗体和中和活性的大小相关。血浆蛋白质组证实了SARS-CoV-2细胞免疫和体液免疫之间的关联，PRNT+患者显示出更高的病毒信号转导分子（SLAMF 1，CD 244，CLEC 4G）。这项工作揭示了儿童的细胞和体液抗SARS-CoV-2反应，这可能会推动未来的疫苗接种试验终点和检疫措施政策。Cotugno等人显示中和抗体影响感染儿童的SARS-CoV-2病毒载量。抗原特异性B和T细胞与病毒中和正相关。这些信息为定义儿童SARS-CoV-2适应性反应提供了基础。
As the global COVID-19 pandemic progresses, it is paramount to gain knowledge on adaptive immunity to SARS-CoV-2 in children to define immune correlates of protection upon immunization or infection. We analyzed anti-SARS-CoV-2 antibodies and their neutralizing activity (PRNT) in 66 COVID-19-infected children at 7 (±2) days after symptom onset. Individuals with specific humoral responses presented faster virus clearance and lower viral load associated with a reduced in vitro infectivity. We demonstrated that the frequencies of SARS-CoV-2-specific CD4+CD40L+ T cells and Spike-specific B cells were associated with the anti-SARS-CoV-2 antibodies and the magnitude of neutralizing activity. The plasma proteome confirmed the association between cellular and humoral SARS-CoV-2 immunity, and PRNT+ patients show higher viral signal transduction molecules (SLAMF1, CD244, CLEC4G). This work sheds lights on cellular and humoral anti-SARS-CoV-2 responses in children, which may drive future vaccination trial endpoints and quarantine measures policies. Cotugno et al. show that neutralizing antibodies affect SARS-CoV-2 viral load in infected children. Antigen-specific B and T cells are positively associated with virus neutralization. This information provides a basis for defining SARS-CoV-2 adaptive responses in children.
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