The effect of glucocorticoids on mitogen-stimulated B-lymphocytes: thymidine incorporation and antibody secretion.
The effect of glucocorticoids on mitogen-stimulated B-lymphocytes: thymidine incorporation and antibody secretion.
复制标题
糖皮质激素对丝裂原刺激的 B 淋巴细胞的影响：胸苷掺入和抗体分泌。
作者：
D. Roess;C. Bellone;M. Ruh;E. Nadel;T. Ruh
用皮质醇敏感种小鼠和相对耐受皮质醇的豚鼠的细胞，研究了糖皮质激素对脂多糖(LPS)刺激的淋巴细胞增殖和分化的影响。B细胞特异性有丝分裂原--脂多糖可使小鼠脾淋巴细胞[~3H]胸腺嘧啶核苷([~3H]TdR)掺入增加5倍。随着糖皮质激素浓度的增加，[~3H]TdR参入率降低。糖皮质激素的生物学效应大小顺序为：曲安奈德；地塞米松；皮质醇；曲安奈德(TA)可使[~3H]TdR掺入从10~(-13)M时的24%下降到10~(-5)M时的75%。皮质酮和黄体酮对脂多糖刺激的小鼠细胞[~3H]TdR掺入均无影响，但过量1000倍的抗糖皮质激素可阻断TA对[~3H]TdR掺入的抑制作用。非促分裂原刺激物。
The effects of glucocorticoids on lipopolysaccharide (LPS)-stimulated lymphocytes undergoing proliferation and differentiation were studied using cells from a corticosensitive species, the mouse, and a relatively corticoresistant species, the guinea pig. A B-cell-specific mitogen, LPS, caused a 5-fold increase in [3H]thymidine ([3H]TdR) incorporation in murine splenic lymphocytes compared to that in nonmitogen-stimulated cells. Increasing concentrations of various glucocorticoids decreased [3H]TdR incorporation. The order of biological effectiveness of the glucocorticoids was triamcinolone acetonide > dexamethasone > cortisol = corticosterone > estradiol. Triamcinolone acetonide (TA) caused decreases in [3H]TdR incorporation ranging from 24% at 10-13 M to 75% at 10-5 M. Neither cortexolone nor progesterone alone affected [3H]TdR incorporation in LPS-stimulated murine cells, but these antiglucocorticoids at a 1000-fold excess blocked the inhibitory effect of TA on [3H]TdR incorporation. Nonmitogen-stimulat...
具核梭杆菌对人外周血 B 淋巴细胞的多克隆激活。
影响因子：
3.1
作者：
Mangan,DF;Lopatin,DE
通讯作者：
Lopatin,DE