Persimmon leaf flavonoid promotes brain ischemic tolerance.
Persimmon leaf flavonoid promotes brain ischemic tolerance.
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DOI：
10.3969/j.issn.1673-5374.2013.28.004
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发表时间：
2013-10-05
影响因子：
6.1
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Miao M;Zhang X;Bai M;Wang L
紫苏叶黄酮已被证明可以增强小鼠脑缺血耐受性，但其作用机制尚不清楚。使用微型夹闭塞双侧颈总动脉以阻断血流10分钟。缺血预处理10 min后，每日灌胃给予柿叶黄酮200、100、50 mg/kg或金纳多20 mg/kg，连续5 d。末次给药后1h，阻断大脑中动脉2 h建立脑缺血再灌注模型。造模后24 h，与未进行缺血预处理或药物干预的脑缺血大鼠相比，缺血预处理大鼠血浆内皮素、血栓调节蛋白和血管性血友病因子水平显著降低，脑组织细胞间粘附分子-1表达显著减少。同时脑组织损伤减轻。缺血预处理结合药物暴露可明显改善上述指标，其中200 mg/kg柿叶黄酮的作用与20 mg/kg金纳多相似。这些结果表明，缺血预处理产生耐受反复严重脑缺血。柿叶黄酮可通过减轻炎症反应和血管内皮损伤来提高缺血耐受性。柿叶黄酮高剂量组与金纳多组具有相同的作用。
Persimmon leaf flavonoid has been shown to enhance brain ischemic tolerance in mice, but its mechanism of action remains unclear. The bilateral common carotid arteries were occluded using a micro clip to block blood flow for 10 minutes. After 10 minutes of ischemic preconditioning, 200, 100, and 50 mg/kg persimmon leaf flavonoid or 20 mg/kg ginaton was intragastrically administered per day for 5 days. At 1 hour after the final administration, ischemia/reperfusion models were estab-lished by blocking the middle cerebral artery for 2 hours. At 24 hours after model establishment, compared with cerebral ischemic rats without ischemic preconditioning or drug intervention, plasma endothelin, thrombomodulin and von Willebrand factor levels significantly decreased and intercel-lular adhesion molecule-1 expression markedly reduced in brain tissue from rats with ischemic pre-conditioning. Simultaneously, brain tissue injury reduced. Ischemic preconditioning combined with drug exposure noticeably improved the effects of the above-mentioned indices, and the effects of 200 mg/kg persimmon leaf flavonoid were similar to 20 mg/kg ginaton treatment. These results indicate that ischemic preconditioning produces tolerance to recurrent severe cerebral ischemia. However, persimmon leaf flavonoid can elevate ischemic tolerance by reducing inflammatory reactions and vascular endothelial injury. High-dose persimmon leaf flavonoid showed an identical effect to ginaton.
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DOI：
10.1046/j.1468-0734.2001.00048.x
发表时间：
2001-12-01
期刊：
Reviews in clinical and experimental hematology
影响因子：
--
作者：
Budde, U;Schneppenheim, R
通讯作者：
Schneppenheim, R
影响因子：
39.3
作者：
Makin, AJ;Blann, AD;Lip, GYH
通讯作者：
Lip, GYH
影响因子：
1.9
作者：
Nagaraja, Tavarekere N.;Knight, Robert A.;Fenstermacher, Joseph D.
通讯作者：
Fenstermacher, Joseph D.
影响因子：
2
作者：
Noto, Takahisa;Furuichi, Yasuhisa;Yanagihara, Takehiko
通讯作者：
Yanagihara, Takehiko
影响因子：
1.1
作者：
Felmeden, DC;Blann, AD;Lip, GYH
通讯作者：
Lip, GYH