Similar diversity of alphaproteobacteria and nitrogenase gene amplicons on two related sphagnum mosses.
Similar diversity of alphaproteobacteria and nitrogenase gene amplicons on two related sphagnum mosses.
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DOI：
10.3389/fmicb.2011.00275
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发表时间：
2011
影响因子：
5.2
通讯作者：
Berg G
中科院分区：
文献类型：
--
作者：
Bragina A;Maier S;Berg C;Müller H;Chobot V;Hadacek F;Berg G
泥炭藓是雨养型湿地的主要植被组成部分。它们拥有特定且多样化的微生物群落，对宿主具有重要功能。为了了解寄主特异性和环境的影响，泥炭藓fallax和泥炭藓angustifolium，两个无性系密切相关的物种，表现出不同的生境偏好方面的营养水平，分析了多方面的方法。通过PCR-单链构象多态性的16 S rRNA和固氮酶编码（nifH）基因获得的微生物指纹是高度相似的泥炭藓物种。通过荧光原位杂交（FISH）结合共聚焦激光扫描显微镜（CLSM）获得的殖民模式的相似性得到证实：Alphaproteobacteria是泥炭藓叶片的透明细胞内的主要殖民者。通过16 S rRNA基因扩增子测序对Alphaproteobacteria进行了更深入的调查，揭示了Acidocella，Acidisphaera，Rhodopila和Phenylobacterium作为两种苔藓的主要属的高度多样性。固氮是泥炭藓伴生细菌的一项重要功能，泥炭藓微生物群落也以类似的方式实现固氮功能。泥炭藓相关微生物群落的NifH文库的特征在于α-变形菌的高多样性和丰度，但也含有其他分类群的多样性扩增子，例如，蓝细菌和三角形变形菌。两种泥炭藓属物种的微生物群落之间的统计学显著差异在任何实验方法中都不能被发现。我们的研究结果表明，同样的密切关系，存在于泥炭藓的物理，形态和化学特性和生态和沼泽生态系统的功能，也连接苔藓植物与其相关的细菌群落。
Sphagnum mosses represent a main vegetation component in ombrotrophic wetlands. They harbor a specific and diverse microbial community with essential functions for the host. To understand the extend of host specificity and impact of environment, Sphagnum fallax and Sphagnum angustifolium, two phylogenetically closely related species, which show distinct habitat preference with respect to the nutrient level, were analyzed by a multifaceted approach. Microbial fingerprints obtained by PCR-single-strand conformation polymorphism of 16S rRNA and nitrogenase-encoding (nifH) genes were highly similar for both Sphagnum species. Similarity was confirmed for colonization patterns obtained by fluorescence in situ hybridization (FISH) coupled with confocal laser scanning microscopy (CLSM): Alphaproteobacteria were the main colonizers inside the hyaline cells of Sphagnum leaves. A deeper survey of Alphaproteobacteria by 16S rRNA gene amplicon sequencing reveals a high diversity with Acidocella, Acidisphaera, Rhodopila, and Phenylobacterium as major genera for both mosses. Nitrogen fixation is an important function of Sphagnum-associated bacteria, which is fulfilled by microbial communities of Sphagna in a similar way. NifH libraries of Sphagnum-associated microbial communities were characterized by high diversity and abundance of Alphaproteobacteria but contained also diverse amplicons of other taxa, e.g., Cyanobacteria and Deltaproteobacteria. Statistically significant differences between the microbial communities of both Sphagnum species could not be discovered in any of the experimental approach. Our results show that the same close relationship, which exists between the physical, morphological, and chemical characteristics of Sphagnum mosses and the ecology and function of bog ecosystems, also connects moss plantlets with their associated bacterial communities.
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影响因子：
5.1
作者：
Opelt, Katja;Chobot, Vladimir;Berg, Gabriele
通讯作者：
Berg, Gabriele
影响因子：
14.9
作者：
Loy A;Maixner F;Wagner M;Horn M
通讯作者：
Horn M
影响因子：
4.8
作者：
Larmola, Tuula;Tuittila, Eeva-Stiina;Fritze, Hannu
通讯作者：
Fritze, Hannu
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
11
作者：
Grube, Martin;Cardinale, Massimiliano;Berg, Gabriele
通讯作者：
Berg, Gabriele