Effect of culture conditions on the kinetics of hydrogen production by photosynthetic bacteria in batch culture

Effect of culture conditions on the kinetics of hydrogen production by photosynthetic bacteria in batch culture
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分批培养条件对光合细菌产氢动力学的影响

DOI:
10.1016/j.ijhydene.2011.04.005
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发表时间:
2011-10
影响因子:
7.2
通讯作者:
Liao, Qiang
Liao, Qiang
中科院分区:
工程技术2区
文献类型:
--
作者:
Li, Jun;Zhu, Xun;Wang, Yong-Zhong;Lee, Duu-Jong;Liao, Qiang

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为优化光发酵产氢工艺,对光发酵产氢的生化动力学特性进行了实验研究和数值模拟。结果表明,在光照强度6000 lux、培养温度30 °C、培养基pH 7.0的条件下,该菌的最大比生长速率为0.26 h-1。这些实验结果还导致了最大比微生物生长速率(μmax)作为光照强度、pH和温度的函数的经验公式。在此基础上,建立了修正的Monod方程沿着,并结合生物量增长、葡萄糖消耗和产氢的动力学方程,对光发酵产氢过程进行了模拟。模拟结果与实验数据吻合较好,表明所建立的动力学模型能够客观地描述不同培养条件下光合细菌的产氢特性。
Biochemical kinetic characteristics of photo-fermentative hydrogen production were experimentally and numerically investigated to optimize the photo-fermentation hydrogen-producing process in this work. It is found that a maximum specific growth rate of 0.26 h−1was achieved under the optimal conditions of illumination intensity 6000 lux, 30 °C culture temperature and pH 7.0 of culture medium. These experimental results also led to an empirical formula of the maximum specific microbial growth rate (μmax) as a function of illumination intensity, pH and temperature. With the empirical formula, the modified Monod equation along with the kinetic equations for biomass growth, glucose consumption and hydrogen production is then developed to simulate the photofermentation hydrogen-producing process. The modeling results are in good agreements with the experimental data, indicating that the developed kinetic models are able to objectively describe the characteristics of hydrogen production by PSB under different culture conditions.
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发表时间: 2007-01-01
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