Primary and secondary indices of stress in the progeny of rainbow trout (Oncorhynchus mykiss) selected for high and low responsiveness to stress
Primary and secondary indices of stress in the progeny of rainbow trout (Oncorhynchus mykiss) selected for high and low responsiveness to stress
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虹鳟鱼 (Oncorhynchus mykiss) 后代的主要和次要应激指数选择对应激的高和低反应
DOI：
10.1111/j.1095-8649.1994.tb01591.x
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发表时间：
1994
影响因子：
2
通讯作者：
J. Morgan
中科院分区：
文献类型：
--
作者：
T. Pottinger;T. Moran;J. Morgan
一系列的混合配子交配进行了雇用鸡蛋和精液从成熟的男性和女性虹鳟鱼选择一个一贯的高或低的应激反应，如应激后血浆皮质醇升高。密切监测后代的发育，并通过两种方法评估高响应亲本的后代和低响应亲本的后代对胁迫的响应。为期14个月，在大约每月的时间间隔，血浆皮质醇升高引起的标准化的禁闭压力，确定在鱼从每个组，其次，在一个场合，血浆皮质醇响应的时间过程中的一个24小时的禁闭期间进行了监测。后代的高响应的父母雪显着更大的皮质醇反应压力比后代的低响应的父母在这两个测试程序。然而，当一个14天的禁闭压力的影响进行了检查，高响应后代显示出更快的血浆皮质醇水平的恢复，而在低响应后代的水平，虽然最初较低，表现出更持久的海拔。为了评估这些观察结果可能的功能影响，测定了循环淋巴细胞数量，这是血细胞补体中免疫学上重要的皮质醇敏感组分。观察到的持续时间的淋巴细胞减少症发病后的限制被认为是与初始的，而不是持续的，皮质醇反应。这些数据表明，通过选择育种来操纵鱼类对胁迫的敏感性是可行的，但仔细选择用于鉴定所需性状的指标是必要的。
A series of pooled gamete matings was carried out employing eggs and milt from mature male and female rainbow trout selected for a consistently high- or low-responsiveness to stress, as indicated by post-stress plasma cortisol elevation. Development of the progeny was closely monitored and the responsiveness to stress of the progeny of high-responding parents and the progeny of low-responding parents was assessed by two methods. For a period of 14 months, at approximately monthly intervals, the plasma cortisol elevation evoked by a standardized confinement stress was determined in fish from each group, and secondly, on one occasion, the time-course of the plasma cortisol response to a 24-h period of confinement was monitored. Progeny of high-responding parents snowed a significantly greater cortisol response to stress than the progeny of low-responding parents during both testing procedures. However, when the effect of a 14-day confinement stress was examined, high-responding progeny showed a more rapid recovery of plasma cortisol levels, while levels in the low-responding progeny, although initially lower, showed a more sustained elevation. To assess the possible functional implications of these observations, circulating lymphocyte numbers, an immunologically important cortisol-sensitive component of the blood cell complement, were determined. The duration of the lymphocytopenia observed following the onset of confinement was found to be related to the initial, not the sustained, cortisol response. These data suggest that manipulation of the sensitivity to stress of fish is feasible by selective breeding, but that careful.choice of the indices employed to identify traits considered desirable is necessary.
影响因子：
4
作者：
RANDWEAVER, M;SWANSON, P;DICKHOFF, WW
通讯作者：
DICKHOFF, WW