A benchmark for chromatin binding measurements in live cells.
A benchmark for chromatin binding measurements in live cells.
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DOI：
10.1093/nar/gks701
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发表时间：
2012-08
影响因子：
14.9
通讯作者：
McNally JG
中科院分区：
文献类型：
--
作者：
Mazza D;Abernathy A;Golob N;Morisaki T;McNally JG
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Live-cell measurement of protein binding to chromatin allows probing cellular biochemistry in physiological conditions, which are difficult to mimic in vitro. However, different studies have yielded widely discrepant predictions, and so it remains uncertain how to make the measurements accurately. To establish a benchmark we measured binding of the transcription factor p53 to chromatin by three approaches: fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and single-molecule tracking (SMT). Using new procedures to analyze the SMT data and to guide the FRAP and FCS analysis, we show how all three approaches yield similar estimates for both the fraction of p53 molecules bound to chromatin (only about 20%) and the residence time of these bound molecules (∼1.8 s). We also apply these procedures to mutants in p53 chromatin binding. Our results support the model that p53 locates specific sites by first binding at sequence-independent sites.
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DOI：
10.1126/science.1202142
发表时间：
2011-04-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Larson DR;Zenklusen D;Wu B;Chao JA;Singer RH
通讯作者：
Singer RH
DOI：
10.1083/jcb.153.7.1341
发表时间：
2001-06-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kimura H;Cook PR
通讯作者：
Cook PR
影响因子：
56.9
作者：
Elf, Johan;Li, Gene-Wei;Xie, X. Sunney
通讯作者：
Xie, X. Sunney
活细胞中 RNA 聚合酶 II 的转录周期。
影响因子：
7.8
作者：
Kimura, Hiroshi;Sugaya, Kimihiko;Cook, Peter R
通讯作者：
Cook, Peter R
影响因子：
4.8
作者：
Hamard, Pierre-Jacques;Lukin, Dana J.;Manfredi, James J.
通讯作者：
Manfredi, James J.