Portable comprehensive two-dimensional micro-gas chromatography using an integrated flow-restricted pneumatic modulator.
Portable comprehensive two-dimensional micro-gas chromatography using an integrated flow-restricted pneumatic modulator.
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DOI：
10.1038/s41378-022-00452-5
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发表时间：
2022
影响因子：
7.9
通讯作者：
Fan, Xudong
中科院分区：
文献类型：
--
作者：
Huang, Xiaheng;Li, Maxwell Wei-hao;Zang, Wenzhe;Huang, Xiaolu;Sivakumar, Anjali Devi;Sharma, Ruchi;Fan, Xudong
二维（2D）气相色谱（GC）提供了与常规一维GC相比增强的蒸气分离能力，并且可用于分析高度复杂的化学样品。我们开发了一种用于便携式综合2D微型GC（μGC）的微制造限流气动调节器（FRPM），其能够在不影响1D分离速度和洗脱液峰形的情况下实现快速2D进样和分离。采用流通式微型光离子化检测器（μ PID）在玻璃纤维增强塑料（FRPM）的进出口处对二维进样特性（如进样峰宽和峰高）进行了全面表征。当2D/1D流速比大于10时，可获得约25 ms的2D注入峰宽。在同一芯片上进一步集成了0.5-m长的2D μ柱，并表征了其性能。最后，我们开发了一个自动化的便携式全面的2D μGC，包括一个10 m OV-1 1D μ柱，一个集成的FRPM与内置的0.5 m聚乙二醇2D μ柱，和两个μ PID。在~5 min内实现了40种挥发性有机化合物的快速分离。通过使用1D和2D μ色谱柱末端的两个μ PID获得的1D和2D色谱图构建混合2D等高线图，这是通过在FRPM中存在流阻实现的。
Two-dimensional (2D) gas chromatography (GC) provides enhanced vapor separation capabilities in contrast to conventional one-dimensional GC and is useful for the analysis of highly complex chemical samples. We developed a microfabricated flow-restricted pneumatic modulator (FRPM) for portable comprehensive 2D micro-GC (μGC), which enables rapid 2D injection and separation without compromising the 1D separation speed and eluent peak profiles. 2D injection characteristics such as injection peak width and peak height were fully characterized by using flow-through micro-photoionization detectors (μPIDs) at the FRPM inlet and outlet. A 2D injection peak width of ~25 ms could be achieved with a 2D/1D flow rate ratio over 10. The FRPM was further integrated with a 0.5-m long 2D μcolumn on the same chip, and its performance was characterized. Finally, we developed an automated portable comprehensive 2D μGC consisting of a 10 m OV-1 1D μcolumn, an integrated FRPM with a built-in 0.5 m polyethylene glycol 2D μcolumn, and two μPIDs. Rapid separation of 40 volatile organic compounds in ~5 min was demonstrated. A hybrid 2D contour plot was constructed by using both 1D and 2D chromatograms obtained with the two μPIDs at the end of the 1D and 2D μcolumns, which was enabled by the presence of the flow resistor in the FRPM.
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