Immunohistological evidence, obtained with monoclonal antibodies, of small intestinal brush border hydrolases in human colon cancers and foetal colons
Immunohistological evidence, obtained with monoclonal antibodies, of small intestinal brush border hydrolases in human colon cancers and foetal colons
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使用单克隆抗体获得的人类结肠癌和胎儿结肠中小肠刷状缘水解酶的免疫组织学证据
DOI：
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发表时间：
1984
影响因子：
6.4
通讯作者：
B. Sordat
中科院分区：
文献类型：
--
作者：
A. Zweibaum;H. Hauri;E. Sterchi;I. Chantret;K. Haffen;Jeanine Bamat;B. Sordat
通过抗人小肠蔗糖酶-异麦芽糖酶（SI）、麦芽糖酶-葡糖淀粉酶（MGA）、乳糖酶（L）、氨肽酶N（APN）和二肽基肽酶IV（DPP-IV）的单克隆抗体，研究了人结肠癌和胎儿结肠中与肠上皮细胞刷状缘膜相关的小肠水解酶的表达。通过间接免疫荧光法，在裸鼠体内生长的6种人结肠癌细胞系（HT-29、Caco-2、SW-480、HRT-18、HCT-8 R和Co-115）肿瘤、27例患者原发性结直肠癌、人胎儿（妊娠16 - 20周）和正常成人小肠和结肠的冷冻切片上观察酶。5个单克隆抗体均与小肠刷状缘结合，而与结肠粘膜刷状缘不结合。抗SI、APN和DPP-IV的抗体也与胎儿结肠的刷状缘、裸鼠HT-29和Caco-2肿瘤的顶端缘以及7/27例患者肿瘤的刷状缘样结构结合。在肿瘤或胎儿结肠中均未观察到MGA和L的结合。在9/11份供试样本中观察到抗SI抗体与肿瘤粘膜上皮刷状缘结合。这些数据表明，一些结肠肿瘤表现出典型的肠细胞分化模式，这是胎儿型，并涉及至少3个刷状缘膜水解酶。因此，小肠水解酶的单克隆抗体可能是鉴定和表征某些分化型结肠肿瘤的重要工具。
The expression of small intestinal hydrolases associated with the enterocyte brush border membrane was studied in human colon cancers and foetal colons, by means of monoclonal antibodies against human small intestinal sucrase‐isomaltase (SI), maltase‐glucoamylase (MGA), lactase (L), aminopeptidase N (APN), and dipeptidylpeptidase IV (DPP‐IV). The enzymes were visualized by indirect immunofluorescence on cryostat sections of tumors developed in nude mice with 6 human colon carcinoma cell lines (HT‐29, Caco‐2, SW‐480, HRT‐18, HCT‐8R, and Co‐115), of 27 primary colorectal carcinomas from patients, and of human foetal (16 to 20 weeks of gestation) and normal adult small intestines and colons. All 5 monoclonals bound to the brush border of the adult small intestine, but not to that of the adult colon mucosa. Antibodies against SI, APN and DPP‐IV also bound to the brush border of the foetal colons, to apical borders in HT‐29 and Caco‐2 tumors in nude mice, and to brush border‐like structures in 7/27 tumors from patients. No binding was observed for MGA and L in either tumors or foetal colons. Binding of anti‐SI antibodies to the brush border of the juxta‐tumoral mucosal epithelium was observed in 9/11 samples tested. These data indicate that some colon tumors exhibit a typical pattern of enterocytic differentiation which is of foetal type and which involves at least 3 brush border membrane hydrolases. Monoclonal antibodies to small intestinal hydrolases may, therefore, be important tools for identification and characterization of some differentiated colonic tumors.
DOI：
10.1016/s0021-9258(19)45389-1
发表时间：
1982-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
通讯作者：
John L. MagnaniS;Bo Nilssong;Manfred BrockhausSV;David Zopfl;Zenon SteplewskiJI;Hilary Koprowskill;V. Ginsburg
单克隆抗体识别胚胎癌前和癌性人类抗原。
DOI：
10.1002/9780470720776.ch12
发表时间：
1983
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
Koprowski,H
通讯作者：
Koprowski,H