Three-dimensional analysis of tumour vascular corrosion casts using stereoimaging and micro-computed tomography.
Three-dimensional analysis of tumour vascular corrosion casts using stereoimaging and micro-computed tomography.
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DOI：
10.1016/j.mvr.2010.03.007
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发表时间：
2010-07
影响因子：
3.1
通讯作者：
Pedley RB
中科院分区：
文献类型：
--
作者：
Folarin AA;Konerding MA;Timonen J;Nagl S;Pedley RB
为了对癌症治疗进行有效的转化研究，我们需要采用反映临床情况的临床前模型。本研究的目的是定量比较人结直肠癌和实验性肿瘤模型的血管结构，以确定血管研究和抗血管治疗的动物模型的适用性。在这项研究中，我们研究了人类临床组织（正常粘膜对照[n = 20]，癌[n = 20]和腺瘤[n = 6]）和小鼠结肠直肠异种移植物（LS 147 T [n = 6]和SW 1222 [n = 6]）中结肠肿瘤血管系统的三维特性。扫描电子显微镜立体成像（SEM）和X射线显微计算机断层扫描（Micro-CT）方法被用于从这些组织的血管腐蚀铸件的3D分析。形态学测量表明，在不同组织中的基本形态存在显著差异。在所研究的异种移植物中，LS147 T比SW 1222更一致地类似于人癌的血管结构。唯一的逆转是血管间距离。虽然SEM立体成像提供了更好的腐蚀铸件的表面细节分辨率，但它得到了全三维显微CT方法的补充。异种移植物和临床肿瘤之间的比较表明，LS 147 T异种移植物与临床肿瘤血管系统有许多相似之处。本研究为临床肿瘤治疗转化研究中如何选择最合适的临床前模型提供了思路。
In order to perform effective translational research for cancer therapy, we need to employ pre-clinical models which reflect the clinical situation. The purpose of this study was to quantitatively compare the vascular architecture of human colorectal cancer and experimental tumour models to determine the suitability of animal models for vascular studies and antivascular therapy. In this study we investigated the three-dimensional properties of colonic tumour vasculature in both human clinical tissues (normal mucosa control [n=20], carcinoma [n=20] and adenoma [n=6]) and murine colorectal xenografts (LS147T [n=6] and SW1222 [n=6]). Scanning Electron Microscope Stereoimaging (SEM) and X-ray Micro-Computed Tomography (Micro-CT) methods were employed for 3D analyses of the vascular corrosion casts from these tissues. Morphological measurements showed that there were significant differences in the underlying morphology in the different tissues. Of the studied xenografts, LS147T is more consistently similar to the vascular architecture of the human carcinoma than SW1222. The only reversal of this is for the inter-vessel distance. While SEM stereoimaging provided better surface detailed resolution of the corrosion casts, it was complimented by the fully 3D micro-CT method. Comparison made between the xenografts and clinical tumours showed that the LS147T xenografts shared many similarities with the clinical tumour vasculature. This study provides insight into how to select the most suitable pre-clinical models for translational studies of clinical cancer therapy.
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影响因子：
8.8
作者：
Konerding, MA;Malkusch, W;Klapthor, B;van Ackern, C;Fait, E;Hill, SA;Parkins, C;Chaplin, DJ;Presta, M;Denekamp, J
通讯作者：
Denekamp, J
影响因子：
11.2
作者：
El Emir, Ethaar;Qureshi, Uzma;Pedley, R. Barbara
通讯作者：
Pedley, R. Barbara
影响因子：
3.1
作者：
Dickie, R.;Bachoo, R. M.;Rogers, R. A.
通讯作者：
Rogers, R. A.
DOI：
10.1177/039463200802100225
发表时间：
2008-04-01
影响因子：
3.5
作者：
Minardi, D.;Lucarini, G.;Muzzonigro, G.
通讯作者：
Muzzonigro, G.
结肠前癌病变和浸润性癌的3D微血管结构。
影响因子：
8.8
作者：
Konerding MA;Fait E;Gaumann A
通讯作者：
Gaumann A