Computer simulations reveal complex distribution of haemodynamic forces in a mouse retina model of angiogenesis.
Computer simulations reveal complex distribution of haemodynamic forces in a mouse retina model of angiogenesis.
复制标题
DOI：
10.1098/rsif.2014.0543
复制
发表时间：
2014-10-06
期刊：
影响因子：
--
通讯作者：
Coveney PV
中科院分区：
文献类型：
--
作者：
Bernabeu MO;Jones ML;Nielsen JH;Krüger T;Nash RW;Groen D;Schmieschek S;Hetherington J;Gerhardt H;Franco CA;Coveney PV
目前，人们对血流动力在控制血管发育过程中所起的作用的了解有限。需要克服的众多障碍之一是能够在厚度只有几微米的船只上以所需的分辨率水平测量这些力量。在这篇文章中，我们提出了一种电子方法，用于计算超出实验测量范围的小鼠视网膜血管系统(一种广泛使用的血管发育动物模型)所经历的血液动力。我们的结果表明，直接从视网膜血管样本重建高分辨率的三维几何模型是可能的，并且格子-Boltzmann算法可以用来获得这些区域的血流动力学的准确估计。我们从出生后第5天和第6天获得的样本生成流动模型。我们的模拟显示在这两种情况下恢复的流动模式之间存在重要差异，包括在存在壁剪切应力(WSS)梯度的区域观察到的回归。我们提出了两种可能的机制来解释P5和P6之间观察到的速度和WSS的增加：(I)在两个时间点之间测量到的典型血管直径的减少和(Ii)修剪过程引发的网络密度的减少。本文开发的方法适用于其他生物医学领域，其中微血管可以成像，但实验流量测量不可用或难以获得。
There is currently limited understanding of the role played by haemodynamic forces on the processes governing vascular development. One of many obstacles to be overcome is being able to measure those forces, at the required resolution level, on vessels only a few micrometres thick. In this paper, we present an in silico method for the computation of the haemodynamic forces experienced by murine retinal vasculature (a widely used vascular development animal model) beyond what is measurable experimentally. Our results show that it is possible to reconstruct high-resolution three-dimensional geometrical models directly from samples of retinal vasculature and that the lattice-Boltzmann algorithm can be used to obtain accurate estimates of the haemodynamics in these domains. We generate flow models from samples obtained at postnatal days (P) 5 and 6. Our simulations show important differences between the flow patterns recovered in both cases, including observations of regression occurring in areas where wall shear stress (WSS) gradients exist. We propose two possible mechanisms to account for the observed increase in velocity and WSS between P5 and P6: (i) the measured reduction in typical vessel diameter between both time points and (ii) the reduction in network density triggered by the pruning process. The methodology developed herein is applicable to other biomedical domains where microvasculature can be imaged but experimental flow measurements are unavailable or difficult to obtain.
登录
查看更多内容
影响因子：
9.8
作者：
Chen Q;Jiang L;Li C;Hu D;Bu JW;Cai D;Du JL
通讯作者：
Du JL
影响因子：
2.4
作者：
Krueger, Timm;Varnik, Fathollah;Raabe, Dierk
通讯作者：
Raabe, Dierk
影响因子：
3.4
作者：
ALM, A;BILL, A
通讯作者：
BILL, A
影响因子：
2.7
作者：
Barber, CB;Dobkin, DP;Huhdanpaa, H
通讯作者：
Huhdanpaa, H
影响因子：
4.4
作者：
Bernabeu, Miguel O.;Nash, Rupert W.;Coveney, Peter V.
通讯作者：
Coveney, Peter V.