Involvements of PCD and changes in gene expression profile during self-pruning of spring shoots in sweet orange (Citrus sinensis).
Involvements of PCD and changes in gene expression profile during self-pruning of spring shoots in sweet orange (Citrus sinensis).
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甜橙（Citrus sinensis）春芽自修剪过程中PCD的参与和基因表达谱的变化
DOI：
10.1186/1471-2164-15-892
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发表时间：
2014-10-13
期刊：
影响因子：
4.4
通讯作者：
Hu CG
中科院分区：
文献类型：
--
作者：
Zhang JZ;Zhao K;Ai XY;Hu CG
柑橘的茎尖在解剖学上不同的修剪区（AZ）处修剪，该修剪区将茎的顶部分成基部和顶端部分（柑橘自修剪）。细胞分离仅发生在AZ，这表明其细胞具有独特的分子调控。虽然有几项研究已经进入形态学方面的自我修剪过程中，潜在的分子机制仍然unknown.ResultsIn这项研究中，程序性细胞死亡（PCD）的标志被确定由TUNEL实验，透射电子显微镜（TEM）和组织化学染色活性氧（ROS）在自修剪的甜橙子的春梢。这些结果表明，PCD是系统的、渐进的，可能在控制柑橘自剪中起重要作用。微阵列分析用于检测自修剪三个阶段的转录组变化，并确定了1，378个差异表达基因。一些基因与PCD有关，而另一些则与细胞壁的生物合成或代谢有关。这些结果强烈表明，在自修剪过程中，分解层激活分解代谢和合成代谢壁修饰途径。此外，自我修剪和相关基因的表达之间有很强的相关性。自修剪在柑橘花芽分化中起着重要作用。因此，本研究采用实时荧光定量PCR和原位杂交技术对橙子中几个关键的开花同源基因和番茄茎尖分生组织（SAM）活性基因进行了研究，结果表明这些基因在SAM和腋生分生组织中优先表达。这将使我们能够更好地理解自修剪和自删除的机制。
BackgroundCitrus shoot tips abscise at an anatomically distinct abscission zone (AZ) that separates the top part of the shoots into basal and apical portions (citrus self-pruning). Cell separation occurs only at the AZ, which suggests its cells have distinctive molecular regulation. Although several studies have looked into the morphological aspects of self-pruning process, the underlying molecular mechanisms remain unknown.ResultsIn this study, the hallmarks of programmed cell death (PCD) were identified by TUNEL experiments, transmission electron microscopy (TEM) and histochemical staining for reactive oxygen species (ROS) during self-pruning of the spring shoots in sweet orange. Our results indicated that PCD occurred systematically and progressively and may play an important role in the control of self-pruning of citrus. Microarray analysis was used to examine transcriptome changes at three stages of self-pruning, and 1,378 differentially expressed genes were identified. Some genes were related to PCD, while others were associated with cell wall biosynthesis or metabolism. These results strongly suggest that abscission layers activate both catabolic and anabolic wall modification pathways during the self-pruning process. In addition, a strong correlation was observed between self-pruning and the expression of hormone-related genes. Self-pruning plays an important role in citrus floral bud initiation. Therefore, several key flowering homologs ofArabidopsisand tomato shoot apical meristem (SAM) activity genes were investigated in sweet orange by real-time PCR andin situhybridization, and the results indicated that these genes were preferentially expressed in SAM as well as axillary meristem.ConclusionBased on these findings, a model for sweet orange spring shoot self-pruning is proposed, which will enable us to better understand the mechanism of self-pruning and abscission.
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