Comparative transcriptional survey between laser-microdissected cells from laminar abscission zone and petiolar cortical tissue during ethylene-promoted abscission in citrus leaves.
Comparative transcriptional survey between laser-microdissected cells from laminar abscission zone and petiolar cortical tissue during ethylene-promoted abscission in citrus leaves.
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DOI：
10.1186/1471-2229-9-127
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发表时间：
2009-10-23
影响因子：
5.3
通讯作者：
Talón M
中科院分区：
文献类型：
--
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
脱落是植物能够脱落器官的细胞分离过程。它对大多数农作物的产量有很大影响。同时，该过程本身也构成了研究细胞分离过程的优秀模型，因为它发生在由特定细胞类型组成的称为脱离区（AZ）的具体区域中。然而，分子方法通常受到 AZ 面积和细胞数量有限的阻碍。因此，对细胞类型分辨率的详细研究对于准确描述该过程并识别生物技术应用的潜在候选基因具有重要意义。已经开发出基于激光捕获显微切割 (LCM) 和 RNA 微提取和扩增的有效分离特定柑橘细胞类型（即层离区 (LAZ) 和叶柄皮质 (Pet) 细胞）的方案。利用微阵列杂交和分析，对来自经过体外 24 小时乙烯处理的柑橘叶外植体的 LAZ 和 Pet 进行比较转录组分析。我们对乙烯处理的 LAZ 中差异表达的基因功能类别的分析揭示了由与蛋白质合成、蛋白质命运、细胞类型分化、发育和转录相关的基因表达主导的激活程序。与细胞壁生物合成和代谢相关的大量基因强烈表明，LAZ 层在脱落过程中激活分解代谢和合成代谢壁修饰途径。此外，不同转录因子家族特定成员的过度表达表明这些基因在柑橘 LAZ 中包含的许多层中有效细胞分离层的分化中发挥着重要作用。宠物中应激相关和防御基因的优先表达表明，该组织可能会被重新编程，以防止器官脱落后的病原体攻击和一般非生物应激。本次调查生成的基于 LCM 的数据代表了对柑橘器官脱落所涉及的主要生物过程和基因的最准确描述。这项研究为乙烯促进的叶片脱落提供了新的分子见解，并确定了用于表征和操纵柑橘器官脱落的新的假定靶基因。
Abscission is the cell separation process by which plants are able to shed organs. It has a great impact on the yield of most crop plants. At the same time, the process itself also constitutes an excellent model to study cell separation processes, since it occurs in concrete areas known as abscission zones (AZs) which are composed of a specific cell type. However, molecular approaches are generally hampered by the limited area and cell number constituting the AZ. Therefore, detailed studies at the resolution of cell type are of great relevance in order to accurately describe the process and to identify potential candidate genes for biotechnological applications. Efficient protocols for the isolation of specific citrus cell types, namely laminar abscission zone (LAZ) and petiolar cortical (Pet) cells based on laser capture microdissection (LCM) and for RNA microextraction and amplification have been developed. A comparative transcriptome analysis between LAZ and Pet from citrus leaf explants subjected to an in-vitro 24 h ethylene treatment was performed utilising microarray hybridization and analysis. Our analyses of gene functional classes differentially represented in ethylene-treated LAZ revealed an activation program dominated by the expression of genes associated with protein synthesis, protein fate, cell type differentiation, development and transcription. The extensive repertoire of genes associated with cell wall biosynthesis and metabolism strongly suggests that LAZ layers activate both catabolic and anabolic wall modification pathways during the abscission program. In addition, over-representation of particular members of different transcription factor families suggests important roles for these genes in the differentiation of the effective cell separation layer within the many layers contained in the citrus LAZ. Preferential expression of stress-related and defensive genes in Pet reveals that this tissue is likely to be reprogrammed to prevent pathogen attacks and general abiotic stresses after organ shedding. The LCM-based data generated in this survey represent the most accurate description of the main biological processes and genes involved in organ abscission in citrus. This study provides novel molecular insight into ethylene-promoted leaf abscission and identifies new putative target genes for characterization and manipulation of organ abscission in citrus.
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影响因子：
6.9
作者：
Agustí J;Merelo P;Cercós M;Tadeo FR;Talón M
通讯作者：
Talón M
影响因子：
1.9
作者：
Burns, JK
通讯作者：
Burns, JK
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
7.4
作者：
Dembinsky, Diana;Woll, Katrin;Hochholdinger, Frank
通讯作者：
Hochholdinger, Frank
影响因子：
7.4
作者：
BenCheikh, W;PerezBotella, J;PrimoMillo, E
通讯作者：
PrimoMillo, E