Protein and mRNA expression of protein kinase C (PKC) in the postmortem brain of bipolar and schizophrenic subjects.
Protein and mRNA expression of protein kinase C (PKC) in the postmortem brain of bipolar and schizophrenic subjects.
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DOI：
10.1016/j.jpsychires.2020.07.019
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发表时间：
2020-11
影响因子：
4.8
通讯作者：
Ren X
中科院分区：
文献类型：
--
作者：
Pandey GN;Rizavi HS;Ren X
蛋白激酶C（PKC）的异常参与了双相情感障碍的病理生理过程。这主要是基于BP患者血小板中PKC的研究。这种异常的PKC活性和亚型是否存在于大脑中尚不清楚。因此，我们确定了PKC活性，PKC亚型在前额叶皮层（PFC），扣带回皮层（CING）和颞叶皮层（TEMP）从BP（n = 19），精神分裂症（SZ）（n = 20）和正常对照组（NC）（n = 25）的表达。脑样本来自哈佛脑库，受试者根据DSM-IV标准进行诊断。使用Western印迹技术测定蛋白质水平，使用实时PCR（qPCR）方法测定mRNA水平。我们发现，有一个显着降低PKC活性的PFC和TEMP从BP科目，但不是从SZ科目获得的胞浆和膜组分。当我们比较PKC同工酶的表达时，我们发现几种同工酶的蛋白和mRNA表达在PFC中显著降低（即，PKCα、PKCβI、PKCβII和PKCε）和TEMP（即，PKCα、PKCβI、PKCβII、PKCε和PKCγ在BP组中表达显著高于对照组（P <0.05）。总的来说，在我们研究的三个脑区中，SZ和NC受试者之间PKC同工酶的mRNA或蛋白表达没有差异。我们的研究结果表明，有一个特定的区域减少某些PKC同工酶的膜和胞浆组分的BP，但不SZ科目。
Abnormalities of protein kinase C (PKC) have been implicated in the pathophysiology of bipolar (BP) illness. This is primarily based on studies of PKC in platelets of BP patients. Whether such abnormalities of PKC activity and isoforms exist in the brain is unclear. We have therefore determined PKC activity, protein and mRNA expression of PKC isoforms in the prefrontal cortex (PFC), cingulate cortex (CING) and temporal cortex (TEMP) from BP (n = 19), schizophrenic (SZ) (n = 20) and normal control (NC) (n = 25) subjects. The brain samples were obtained from the Harvard Brain Bank, and the subjects were diagnosed according to DSM-IV criteria. Protein levels were determined using Western blot technique and mRNA levels were determined using real-time PCR (qPCR) method. We found that there was a significant decrease in the PKC activity in the cytosol and membrane fractions of PFC and TEMP obtained from BP subjects but not from SZ subjects. When we compared the expression of PKC isozymes, we found that the protein and mRNA expression of several isozymes was significantly decreased in the PFC (i.e., PKCα, PKCβI, PKCβII and PKCε) and TEMP (i.e., PKCα, PKCβI, PKCβII, PKCε and PKCγ) of BP subjects, but not in the CING. Overall, there was no difference in the mRNA or protein expression of PKC isozymes between SZ and NC subjects in any of the three brain areas we studied. Our results show that there is a region-specific decrease of certain PKC isozymes in the membrane and cytosol fractions of BP but not SZ subjects.
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DOI：
10.1083/jcb.111.4.1559
发表时间：
1990-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Deloulme JC;Janet T;Au D;Storm DR;Sensenbrenner M;Baudier J
通讯作者：
Baudier J
影响因子：
5.1
作者：
Abrial, Erika;Lucas, Guillaume;Lambas-Senas, Laura
通讯作者：
Lambas-Senas, Laura
影响因子：
30.8
作者：
Ferreira, Manuel A. R.;O'Donovan, Michael C.;Meng, Yan A.;Jones, Ian R.;Ruderfer, Douglas M.;Jones, Lisa;Fan, Jinbo;Kirov, George;Perlis, Roy H.;Green, Elaine K.;Smoller, Jordan W.;Grozeva, Detelina;Stone, Jennifer;Nikolov, Ivan;Chambert, Kimberly;Hamshere, Marian L.;Nimgaonkar, Vishwajit L.;Moskvina, Valentina;Thase, Michael E.;Caesar, Sian;Sachs, Gary S.;Franklin, Jennifer;Gordon-Smith, Katherine;Ardlie, Kristin G.;Gabriel, Stacey B.;Fraser, Christine;Blumenstiel, Brendan;Defelice, Matthew;Breen, Gerome;Gill, Michael;Morris, Derek W.;Elkin, Amanda;Muir, Walter J.;McGhee, Kevin A.;Williamson, Richard;MacIntyre, Donald J.;MacLean, Alan W.;Clair, David St;Robinson, Michelle;Van Beck, Margaret;Pereira, Ana C. P.;Kandaswamy, Radhika;McQuillin, Andrew;Collier, David A.;Bass, Nicholas J.;Young, Allan H.;Lawrence, Jacob;Ferrier, I. Nicol;Anjorin, Adebayo;Farmer, Anne;Curtis, David;Scolnick, Edward M.;McGuffin, Peter;Daly, Mark J.;Corvin, Aiden P.;Holmans, Peter A.;Blackwood, Douglas H.;Gurling, Hugh M.;Owen, Michael J.;Purcell, Shaun M.;Sklar, Pamela;Craddock, Nick
通讯作者：
Craddock, Nick
影响因子：
5.4
作者：
Hahn, C G;Friedman, E
通讯作者：
Friedman, E
影响因子：
--
作者：
FEIGHNER, JP;WOODRUFF, RA;GUZE, SB
通讯作者：
GUZE, SB