Sprout vacuum-infiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species
Sprout vacuum-infiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species
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芽芽真空渗透：一种简单有效的农业接种方法，用于多种茄科物种病毒诱导的基因沉默
DOI：
10.1007/s00299-012-1285-1
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发表时间：
2012-06
影响因子：
6.2
通讯作者：
Luo YB
中科院分区：
文献类型：
--
作者：
Yan HX;Fu DQ（同享第一作者）;Zhu BZ;Liu HP;Shen XY;Luo YB
病毒诱导的基因沉默（VIGS）是一种鉴定植物基因功能的有效技术。烟草脆裂病毒（TRV）介导的VIGS已被广泛应用于许多植物。为了克服现有农杆菌接种方法的局限性，本文报道了一种简便有效的病毒基因沉默农杆菌接种方法--芽真空渗入法。我们利用芽真空渗透法成功地沉默了番茄、茄子、辣椒和本氏烟草四种重要茄科作物中八氢番茄红素脱氢酶和镁原卟啉螯合酶基因的表达。基因沉默的表型在1周龄的植株中是明显的。与其他技术如叶片渗透或农业灌溉相比，该方法简单、成本低且快速。这对于研究植物生长早期的基因功能可能更有实际意义。SVI的一个重要方面是它将在未来用于高通量VIGS筛选。SVI是一种简单、低成本的VIGS农杆菌接种方法，它将克服现有接种方法的局限性，为大规模的VIGS cDNA文库分析提供便利。这对于研究植物生长早期表达基因的功能和高通量VIGS筛选具有实用价值。
Virus-induced gene silencing (VIGS) is a robust technique for identifying the functions of plant genes. Tobacco rattle virus (TRV)-mediated VIGS has been commonly used in many plants. In order to overcome the limitations of existing agroinoculation methods, we report an easy and effective method of agroinoculation for virus-induced gene silencing—sprout vacuum-infiltration (SVI). Using sprout vacuum-infiltration, we have successfully silenced the expression of phytoene desaturase and Mg-protoporphyrin chelatase genes in four important solanaceous crops, including tomato, eggplant, pepper, andNicotiana benthamiana. The gene-silenced phenotypes are conspicuous in 1-week-old plants. The method is simple, low cost and rapid compared to other techniques such as leaf infiltration or agrodrench. It may be more practical for studying gene function in the early stages of plant growth. An important aspect of SVI is that it will be used for high-throughput VIGS screens in the future. SVI will be an effective tool to overcome the limitations of current inoculation methods and to facilitate large-scale VIGS analysis of cDNA libraries.Key messageSVI is a simple, low cost agroinoculation method for VIGS. It is practical for studying the function of genes expressed in early stages of plant growth and high-throughput VIGS screens.
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影响因子：
5.1
作者：
Liu, Y;Nakayama, N;Dinesh-Kumar, SP
通讯作者：
Dinesh-Kumar, SP
影响因子：
--
作者：
S. P. Dinesh-Kumar;R. Anandalakshmi;R. Marathe;Michael Schiff;Yule Liu
通讯作者：
S. P. Dinesh-Kumar;R. Anandalakshmi;R. Marathe;Michael Schiff;Yule Liu
影响因子：
7.2
作者：
Ratcliff, F;Martin-Hernandez, AM;Baulcombe, DC
通讯作者：
Baulcombe, DC
影响因子：
1.2
作者：
Gao, Xiquan;Britt, Robert C.;He, Ping
通讯作者：
He, Ping
影响因子：
7.2
作者：
Brigneti, G;Martín-Hernández, AM;Jones, JDG
通讯作者：
Jones, JDG