Morphophysiological study on the divergent projection of axon collaterals of medial vestibular nucleus neurons in the cat
Morphophysiological study on the divergent projection of axon collaterals of medial vestibular nucleus neurons in the cat
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猫内侧前庭核神经元轴突侧枝发散投射的形态生理学研究
DOI：
10.1007/bf00239407
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发表时间：
2004
影响因子：
2
通讯作者：
J. Yokota
中科院分区：
文献类型：
--
作者：
N. Isu;J. Yokota
摘要(1)在去大脑猫的前庭内侧核(MVN)细胞外记录到单个神经元的棘波，通过观察刺激同侧前庭神经后它们对水平旋转和单突触激活的反应，在功能上被鉴定为次级I型神经元。这些神经元的轴突投射是通过它们对刺激对侧外展神经核、脊髓和上行和下行MLF的逆向反应来检测的。(2)向对侧展神经核投射轴突的I型前庭次级神经元，几乎都是在OBEX水平从MLF下行逆行激活的。其中近一半的神经元将其侧枝轴突投射到脊髓的C_1节段，约三分之一投射到动眼神经复合体附近的上行MLF。(3)下行侧支平均传导速度为29m/S，上行侧支平均传导速度为30m/S。(4)对侧外展神经核和脊髓灰质C2-C3区逆行微刺激的系统追踪结果表明，单个I型前庭神经元的侧枝轴突发出外展神经核内的局部分支和颈上灰质的运动神经元池。用辣根过氧化物酶(HRP)进行轴突内染色，证实了颈运动神经元池内终支的存在。(5)投射到对侧外展神经核和脊髓的神经元位于MVN吻侧腹外侧部相当局限的区域。投射到对侧外展神经核而不投射到脊髓的神经元位于MVN腹外侧吻尾侧较宽的区域。投射到脊髓而不投射到对侧外展神经核的神经元位于吻尾方向最宽的区域，几乎覆盖了MVN吻侧半部的整个范围。
Summary(1) Spikes of single neurons were extracellularly recorded in the medial vestibular nucleus (MVN) in decerebrate cats and were functionally identified as secondary type I neurons by observing their responses to horizontal rotation and monosynaptic activation after stimulation of the ipsilateral vestibular nerve. Axonal projection of these neurons was examined by their antidromic responses to stimulation of the contralateral abducens nucleus, the spinal cord, and the ascending and descending MLF. (2) Almost all secondary type I vestibular neurons which sent their axon to the contralateral abducens nucleus were antidromically activated from the descending MLF at the level of the obex as well. Nearly half of these neurons sent their collateral axon to the level of C1 segment in the spinal cord and approximately one third to the ascending MLF close to the oculomotor complex. (3) The mean conduction velocity was 29 m/s for descending collateral axons and 30 m/s for ascending collateral axons. (4) Systematic tracking for antidromic microstimulation in the contralateral abducens nucleus and spinal gray matter at C2–C3 suggested that collateral axons of single type I vestibular neurons gave off local branches in the abducens nucleus and the motoneuron pool in the upper cervical gray matter. Existence of terminal branches in the neck motoneuron pool was confirmed by intraaxonal staining with horseradish peroxidase (HRP). (5) Neurons which projected to both the contralateral abducens nucleus and the spinal cord were located in a fairly localized region in the ventrolateral part of the rostral MVN. Neurons which projected to the contralateral abducens nucleus and not to the spinal cord were located in a rostrocaudally wider area in the ventrolateral MVN. Neurons projecting to the spinal cord and not to the contralateral abducens nucleus were located in the widest area in the rostrocaudal direction, covering almost the whole extent of the rostral half of the MVN.
影响因子：
3.2
作者：
GRAHAM, RC;KARNOVSKY, MJ
通讯作者：
KARNOVSKY, MJ