Humoral immune response in disease‐free advanced melanoma patients after vaccination with melanoma‐associated gangliosides
Humoral immune response in disease‐free advanced melanoma patients after vaccination with melanoma‐associated gangliosides
复制标题
无病晚期黑色素瘤患者接种黑色素瘤相关神经节苷脂疫苗后的体液免疫反应
DOI：
10.1002/ijc.2910490616
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发表时间：
1991
影响因子：
6.4
通讯作者：
J. Doré
中科院分区：
文献类型：
--
作者：
J. Portoukalian;S. Carrel;J. Doré
Several studies have shown that melanoma‐associated gangliosides are immunogenic in melanoma patients and that antibodies against them have a favorable prognostic effect. Our study aims at characterizing the humoral immune response in disease‐free, advanced melanoma patients vaccinated with a total ganglioside fraction extracted from pooled metastases of human melanoma, containing as major gangliosides GM3 and GD3, and as minor ones GM2 and GD2. Prior to vaccination, all patients were made disease‐free by surgical removal of skin, lymph‐node or other distant metastases. Repeated vaccinations were carried out intradermally with gangliosides either in the native form in buffered solution, or in the form of liposomes. Serum samples were collected at regular intervals and assayed by ELISA for the presence of specific IgG and IgM antiganglioside antibodies. Selected samples were tested by immunostaining on thin‐layer plates to specify the ganglioside species involved in the reactivity. Out of 32 evaluable patients, 17 presented a significant increase in antibody titer, mostly of the IgG isotype, which was maximal between 2 and 4 months after starting injections of gangliosides, and gradually disappeared within 1 year. No significant difference could be seen between the group of 20 patients treated with native gangliosides and the group of 12 vaccinated with the gangliosides in liposomes. All gangliosides seemed to be immunogenic, but GM2 and GD2 were somewhat more reactive. The disease‐free intervals for the patients who showed an antibody response to the treatment were significantly higher (p < 0001) than those of the non‐responding group, as compared by the Kaplan‐Meier method.
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神经节苷脂 O-乙酰基-GD3 的共同表位，在主动特异性免疫治疗后，黑色素瘤患者的抗体可识别 GD3。
影响因子：
11.2
作者：
Ravindranath,MH;Morton,DL;Irie,RF
通讯作者：
Irie,RF
携带神经节苷脂GM3内部图像的抗独特型单克隆抗体。
DOI：
10.1093/jnci/82.22.1757
发表时间：
1990
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Yamamoto,S;Yamamoto,T;Saxton,RE;Hoon,DS;Irie,RF
通讯作者：
Irie,RF
影响因子：
3.2
作者：
Zhou Z;Mondal M;Liao G;Guo Z
通讯作者：
Guo Z
DOI：
10.1073/pnas.80.17.5392
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
TAI, T;PAULSON, JC;IRIE, RF
通讯作者：
IRIE, RF