Pentad: A reproducible cytoarchitectonic protocol and its application to parcellation of the human hippocampus.
Pentad: A reproducible cytoarchitectonic protocol and its application to parcellation of the human hippocampus.
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DOI：
10.3389/fnana.2023.1114757
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发表时间：
2023
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
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海马体对于学习和记忆至关重要，是多种疾病的目标。神经影像学方法经常使用海马亚区体积作为神经退行性变的标准测量，从而使它们成为研究的重要生物标志物。总的来说，组织学分区研究存在各种分歧、差异和遗漏。本研究旨在通过建立第一个基于组织学的分割协议来推进海马亚区分割领域，应用于 n = 22 个人类海马样本。该协议重点关注在人类海马体锥体层中观察到的五种细胞特征。我们将这种方法称为pentad协议。这些特征是：嗜色性、神经元大小、堆积密度、聚类和共线性。包括的子域有 CA1、CA2、CA3、CA4、prosubiculum、subiculum、presubiculum、parasubiculum，以及内侧（uncal）子域 Subu、CA1u、CA2u、CA3u 和 CA4u。我们还在冠状面上建立了海马体的九个不同的前后水平，以记录轴尾差异。应用 pentad 协议，我们将 22 个样本中的 9 个级别的 13 个子字段进行了划分。我们发现 CA1 具有最小的神经元，CA2 显示出高度的神经元聚类，而 CA3 显示了 CA 区域中最多的共线神经元。前下托和下托之间的边界呈阶梯状，并且副下托比前下托具有更大的神经元。我们还证明了 CA4 和 prosubiculum 作为单独的子域存在的细胞结构证据。该协议是全面的、严格的，并提供大量样本、海马亚区和前后冠状水平。 pentad 协议利用人类海马子场分区的黄金标准方法。
The hippocampus is integral for learning and memory and is targeted by multiple diseases. Neuroimaging approaches frequently use hippocampal subfield volumes as a standard measure of neurodegeneration, thus making them an essential biomarker to study. Collectively, histologic parcellation studies contain various disagreements, discrepancies, and omissions. The present study aimed to advance the hippocampal subfield segmentation field by establishing the first histology based parcellation protocol, applied to n = 22 human hippocampal samples. The protocol focuses on five cellular traits observed in the pyramidal layer of the human hippocampus. We coin this approach the pentad protocol. The traits were: chromophilia, neuron size, packing density, clustering, and collinearity. Subfields included were CA1, CA2, CA3, CA4, prosubiculum, subiculum, presubiculum, parasubiculum, as well as the medial (uncal) subfields Subu, CA1u, CA2u, CA3u, and CA4u. We also establish nine distinct anterior-posterior levels of the hippocampus in the coronal plane to document rostrocaudal differences. Applying the pentad protocol, we parcellated 13 subfields at nine levels in 22 samples. We found that CA1 had the smallest neurons, CA2 showed high neuronal clustering, and CA3 displayed the most collinear neurons of the CA fields. The border between presubiculum and subiculum was staircase shaped, and parasubiculum had larger neurons than presubiculum. We also demonstrate cytoarchitectural evidence that CA4 and prosubiculum exist as individual subfields. This protocol is comprehensive, regimented and supplies a high number of samples, hippocampal subfields, and anterior-posterior coronal levels. The pentad protocol utilizes the gold standard approach for the human hippocampus subfield parcellation.
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影响因子：
3.5
作者：
Daugherty AM;Bender AR;Raz N;Ofen N
通讯作者：
Ofen N
影响因子：
6.3
作者：
Bartsch, Thorsten;Doehring, Juliane;Jansen, Olav
通讯作者：
Jansen, Olav
影响因子：
5.7
作者：
Adler DH;Pluta J;Kadivar S;Craige C;Gee JC;Avants BB;Yushkevich PA
通讯作者：
Yushkevich PA
人类海马子场中的多素模式分析。
影响因子：
2.9
作者：
Bonnici HM;Chadwick MJ;Kumaran D;Hassabis D;Weiskopf N;Maguire EA
通讯作者：
Maguire EA
影响因子：
11
作者：
Butt UJ;Steixner-Kumar AA;Depp C;Sun T;Hassouna I;Wüstefeld L;Arinrad S;Zillmann MR;Schopf N;Fernandez Garcia-Agudo L;Mohrmann L;Bode U;Ronnenberg A;Hindermann M;Goebbels S;Bonn S;Katschinski DM;Miskowiak KW;Nave KA;Ehrenreich H
通讯作者：
Ehrenreich H