EWVDV-Mediated Platelet-Targeting Nanoparticles for the Multimodal Imaging of Thrombi at Different Blood Flow Velocities
EWVDV-Mediated Platelet-Targeting Nanoparticles for the Multimodal Imaging of Thrombi at Different Blood Flow Velocities
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EWVDV 介导的血小板靶向纳米颗粒用于不同血流速度下血栓的多模态成像
DOI：
10.2147/ijn.s233968
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Dajing Guo
中科院分区：
文献类型：
--
作者：
Jie Xu;Jun Zhou;Yixin Zhong;Yu Zhang;Man Ye;Jingxin Hou;Zhigang Wang;Haitao Ran;Jia Liu;Dajing Guo
背景近年来，血栓分子探针的研究报道较多，但其体内靶向效果并不理想，这可能与体内血流速度有关。因此，靶向效应与血流速度之间的关系值得解释。方法与材料在本研究中，我们构建了一个血小板靶向纳米粒子（NP）的基础上EWVDV靶向P-选择素结合相变材料全氟己烷和印度墨水，以实现血栓的多模态成像。模拟体内血流速度，研究了不同流速下纳米粒对兔血栓的靶向作用。结果成功制备了具有低强度聚焦超声辐照相变能力的纳米粒子，并对活化血小板具有高亲和力。在体外，低流速（20 cm/s）几乎不影响纳米粒子的靶向效果，而中等流速（40 cm/s）与静态流体（0 cm/s）相比，靶向血栓的纳米粒子数量减少了52.6%。高流速（60 cm/s）大大降低了83.5%的纳米粒子的靶向效果。结论该结果可为根据血流速度设计不同部位、不同血管类型的血栓靶向纳米粒提供参考，为体内实验奠定基础。
Background There have been many recent reports of molecular probes for thrombi but with unsatisfactory in vivo targeting effects, which could be related to the blood flow velocity in vivo. Therefore, it is worth explaining the relationship between the targeting effect and the blood flow velocity. Methods and Materials In this study, we constructed a platelet-targeting nanoparticle (NP) based on EWVDV for targeting P-selectin combined with the phase transition material perfluorohexane and India ink to achieve the multimodal imaging of thrombi. We studied the targeting effect of the NPs for rabbit blood thrombi under different flow velocities simulating blood flow velocities in vivo. Results The results show the successful fabrication of NPs with the ability to undergo a phase transition via low-intensity focused ultrasound irradiation to achieve ultrasound imaging and with a high binding affinity for activated platelets. In vitro, low flow velocities (20 cm/s) hardly affected the targeting effect of the NPs, while moderate flow velocities (40 cm/s) reduced the number of NPs that target thrombi by 52.6% comparing to static fluid (0 cm/s). High flow velocities (60 cm/s) greatly reduced the targeting effect of the NPs by 83.5%. Conclusion These results can serve as a reference for the design of NPs targeting thrombi at different sites and in different blood vessel types according to the blood flow velocity, thereby establishing a foundation for in vivo experiments.
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影响因子：
4.4
作者：
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影响因子：
4.8
作者：
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通讯作者：
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