Phenotypic characterization of adenomyosis occurring at the inner and outer myometrium.
Phenotypic characterization of adenomyosis occurring at the inner and outer myometrium.
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DOI：
10.1371/journal.pone.0189522
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kobayashi H
中科院分区：
文献类型：
--
作者：
Kishi Y;Shimada K;Fujii T;Uchiyama T;Yoshimoto C;Konishi N;Ohbayashi C;Kobayashi H
目的：评估发生在子宫内侧或外侧肌层的子宫腺肌病纤维化过程的表型特征。8例子宫腺肌病发生在子宫内肌层(亚型)，10例子宫腺肌病发生在子宫外肌层(亚型II)，10例正常对照。用细胞骨架蛋白、I型和III型胶原、转化生长因子-β及其信号分子对平滑肌细胞进行免疫组织化学研究。I型胶原在子宫腺肌病病灶的细胞外基质中表达增加。在正常子宫组织中，SMC分化标志物蛋白(Desmin、Smoothelin、Myosin Heavy Chain(MHC))在子宫内侧肌层不表达或仅少量表达，而在外侧肌层均有明显表达。在这两种类型的子宫腺肌病病灶中，结蛋白、Smoothelin和MHC通常在腺体邻近区域呈阴性染色。非肌型肌球蛋白IIB、转化生长因子-β和磷酸化转化生长因子-βI型受体仅在II型子宫腺肌病的SMC中显著表达。II型子宫腺肌病的Smad3/2比值显著高于I型子宫腺肌病。正常子宫内侧肌层由未分化的SMC表型组成，而外侧肌层由终末分化的SMC组成。在子宫腺肌病的发展过程中，各种纤维化过程已被提出。纤维化相关蛋白的不同表达模式已被证明与子宫腺肌病亚型的不同有关。
To estimate the phenotypic characterization of fibrotic process in adenomyosis occurring at the inner or the outer myometrium. Eight cases of adenomyosis occurring at the inner myometrium (Subtype I) and 10 cases of adenomyosis occurring at the outer myometrium (Subtype II), and 10 normal counterparts were used in this study. A immunohistochemical study for smooth muscle cells (SMCs) was performed using cytoskeletal proteins, Type I and III collagen, TGF-β and its signaling molecules. An increased expression of Type I collagen was observed in the extracellular matrix of adenomyotic foci. In normal uteri, immunostaining of SMC differentiation marker proteins (Desmin, Smoothelin, Myosin heavy chain (MHC)) were absent or only found in low numbers at the inner myometrium, while all of these marker proteins were clearly stained at the outer myometrium. In both types of adenomyotic foci, Desmin, Smoothelin, and MHC commonly showed a negative staining at the adjacent area to the glands. A significant staining of Non-muscle myosin IIB, TGF-β, and phosphorylated TGF-β type I receptors were found only at the SMCs of Subtype II adenomyosis. The Smad3/2 ratio of Subtype II adenomyosis was significantly higher than that of Subtype I. The inner myometrium of normal uteri was composed of undifferentiated phenotypes of SMCs, while the outer myometrium was composed of terminally differentiated SMCs. Various fibrotic processes have been suggested in the development of uterine adenomyosis. Distinct expression patterns of fibrosis related proteins have been shown to be implicated with differences in the subtypes of adenomyosis.
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影响因子：
3.7
作者：
Shi X;DiRenzo D;Guo LW;Franco SR;Wang B;Seedial S;Kent KC
通讯作者：
Kent KC
影响因子：
3.7
作者：
Young VJ;Brown JK;Saunders PT;Duncan WC;Horne AW
通讯作者：
Horne AW
影响因子：
64.8
作者：
WRANA, JL;ATTISANO, L;MASSAGUE, J
通讯作者：
MASSAGUE, J
影响因子：
2.9
作者：
Ibrahim, Mohamed Gamal;Sillem, Martin;Mechsner, Sylvia
通讯作者：
Mechsner, Sylvia
影响因子：
5.5
作者：
Meyer, Gretchen A.;Lieber, Richard L.
通讯作者：
Lieber, Richard L.