Sensitivity of Polymerase Chain Reaction Assay for Rickettsia tsutsugamushi in Patients' Blood Samples
Sensitivity of Polymerase Chain Reaction Assay for Rickettsia tsutsugamushi in Patients' Blood Samples
复制标题
聚合酶链反应测定患者血液样本中恙虫病立克次体的灵敏度
DOI：
10.1111/j.1348-0421.1992.tb02118.x
复制
发表时间：
1992
影响因子：
2.6
通讯作者：
T. Oshikawa
中科院分区：
文献类型：
--
作者：
K. Murai;N. Tachibana;A. Okayama;E. Shishime;K. Tsuda;T. Oshikawa
建立了检测恙虫病立克次体的套式聚合酶链反应（PCR）方法。恙虫病）DNA，并测定其敏感性。引物选自Karp菌株的58-kDa组特异性抗原基因的DNA序列。从感染立克次体的BS-C-1细胞中提取的1.0 μg DNA中，可检测到立克次体DNA的靶序列，为对应于88 bp的条带。恙虫病。不仅在同源Karp菌株中观察到立克次体特异性条带，而且在四种异源菌株中也观察到立克次体特异性条带：另外两种参考菌株（Gilliam和Kato）和宫崎地区流行的两种原型菌株（Irie和Hirano）。用这种方法检测的最小拷贝数估计为5个立克次体。发病后2 - 11天就诊的恙虫病患者的所有9份外周血单核细胞样本均检测为立克次体DNA阳性。本研究建立的PCR检测方法可作为恙虫病的特异性诊断工具，特别是在急性期早期。
We developed a nested polymerase chain reaction (PCR) method to detect Rickettsia tsutsugamushi (R. tsutsugamushi) DNA and determined its sensitivity. Primers were selected from the DNA sequence of the 58‐kDa group‐specific antigen gene of the Karp strain. The target sequence of rickettsial DNA was detectable as the band corresponding to 88 bp in 1.0 μg of the DNA extracted from BS‐C‐1 cells infected with R. tsutsugamushi. Rickettsia‐specific bands were observed not only for the homologous Karp strain, but also for four heterologous strains: two other reference strains (Gilliam and Kato) and two prototype strains prevalent in Miyazaki district (Irie and Hirano). The minimum copy number detectable by this method was estimated to be five rickettsiae. All of nine peripheral blood mononuclear cell samples from patients with tsutsugamushi disease who were seen 2‐11 days after disease onset tested positive for rickettsial DNA. The PCR assay method presented here could be a specific diagnostic tool for tsutsugamushi disease, especially in its early acute stage.
Ohashi，Norio：“来自恙虫病立克次体的类型特异性抗原（TSA）编码基因（tsg56）的分子克隆和核苷酸序列”基因。
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--
日本立克次体病。
DOI：
--
发表时间：
1988
期刊：
The Japanese journal of experimental medicine
影响因子：
--
作者：
Akiyoshi Kawamura;H. Tanaka
通讯作者：
H. Tanaka