Anorexic effects of intra-VTA leptin are similar in low-fat and high-fat-fed rats but attenuated in a subgroup of high-fat-fed obese rats.
Anorexic effects of intra-VTA leptin are similar in low-fat and high-fat-fed rats but attenuated in a subgroup of high-fat-fed obese rats.
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DOI：
10.1016/j.pbb.2012.10.012
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发表时间：
2013-01
影响因子：
3.6
通讯作者：
Corrie, Lu W.
中科院分区：
文献类型：
--
作者：
Bruijnzeel, Adrie W.;Qi, Xiaoli;Corrie, Lu W.
瘦素是一种肥胖激素，在调节食物摄入和能量平衡中起重要作用。本研究探讨了高脂（HF）和低脂、高糖（LF）饮食对大鼠腹侧被盖区（VTA）瘦素敏感性的影响。动物暴露于HF或LF饮食16周。然后研究腹侧被盖区内瘦素（150和500 ng/侧，单侧剂量）对食物摄入和体重的影响，同时动物维持HF或LF饮食。长期暴露于HF或LF饮食导致这些组中相似的体重增加。HF喂养的动物比LF喂养的动物消耗的食物量更少，但两组消耗的卡路里量相同。在HF和LF喂养的动物中，双侧腹侧被盖区给予瘦素可降低摄食量（72 h）和体重（48 h），降低程度相似。当HF喂养的动物按体重增加排序时，显示饮食诱导的肥胖大鼠（HF喂养的DIO，体重增加的上四分位数）对瘦素对食物摄入和体重的影响的敏感性低于饮食抵抗大鼠（HF喂养的DR，体重增加的下四分位数）。用荧光Cy 3标记的瘦素的对照实验表明瘦素没有扩散超出腹侧被盖区的边界。这项研究表明，腹侧被盖区的瘦素敏感性在暴露于HF或LF饮食的动物中是相同的。然而，HF喂养的DIO大鼠的腹侧被盖区瘦素的作用不如HF喂养的DR大鼠敏感。当暴露于HF饮食时，腹侧被盖区的瘦素抵抗可能导致暴饮暴食和体重增加。
Leptin is an adiposity hormone that plays an important role in regulating food intake and energy homeostasis. This study investigated the effects of a high-fat (HF) and a low-fat, high-carbohydrate/sugar (LF) diet on leptin sensitivity in the ventral tegmental area (VTA) in rats. The animals were exposed to a HF or LF diet for 16 weeks. Then the effects of intra-VTA leptin (150 and 500 ng/side, unilateral dose) on food intake and body weights were investigated while the animals were maintained on the HF or LF diet. Long-term exposure to the HF or LF diet led to similar body weight gain in these groups. The HF-fed animals consumed a smaller amount of food by weight than the LF-fed animals but both groups consumed the same amount of calories. The bilateral administration of leptin into the VTA decreased food intake (72 h) and body weights (48 h) to a similar degree in the HF and LF-fed animals. When the HF-fed animals were ranked by body weight gain it was shown that the diet-induced obese rats (HF-fed DIO, upper quartile for weight gain) were less sensitive to the effects of leptin on food intake and body weights than the diet-resistant rats (HF-fed DR, lower quartile for weight gain). A control experiment with fluorescent Cy3-labeled leptin showed that leptin did not spread beyond the borders of the VTA. This study indicates that leptin sensitivity in the VTA is the same in animals that are exposed to a HF or LF diet. However, HF-fed DIO rats are less sensitive to the effects of leptin in the VTA than HF-fed DR rats. Leptin resistance in the VTA might contribute to overeating and weight gain when exposed to a HF diet.
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