New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli.
New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. coli.
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DOI：
10.1371/journal.pone.0029481
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Curtiss R 3rd
中科院分区：
文献类型：
--
作者：
Mellata M;Maddux JT;Nam T;Thomson N;Hauser H;Stevens MP;Mukhopadhyay S;Sarker S;Crabbé A;Nickerson CA;Santander J;Curtiss R 3rd
肠外致病性E.大肠杆菌（ExPEC），包括禽致病性E.大肠杆菌（APEC），对人类和动物健康构成相当大的威胁，疾病造成巨大的经济损失。APEC菌株χ7122（O 78 ∶K80∶H9）含有3个大质粒[pChi 7122 -1（IncFIB/FIIA-FIC）、pChi 7122 -2（IncFII）和pChi 7122 -3（IncI 2）]和一个小质粒pChi 7122 -4（ColE 2-like），多年来一直被用作模式菌株来研究ExPEC致病性和人畜共患病潜力的分子机制。我们之前对质粒pChi 7122 -1进行了测序和表征，并确定了其在系统性APEC感染中的重要性;然而，其他pChi 7122质粒的作用仍然不明确。在此，我们给出了其余pChi 7122质粒的序列，证实pChi 7122 -2和pChi 7122 -3分别编码ABC铁转运系统（eitABCD）和推定的IV型菌毛，而pChi 7122 -4是隐蔽质粒。新的特征也被发现，包括pChi 7122 -2上的基因簇，这是不存在于其他E。大肠杆菌菌株，但在沙门氏菌血清型中发现，并预测编码糖分解代谢途径。体外评价的APEC × 7122衍生菌株与三个大质粒，无论是单独或组合，提供了新的见解质粒的作用，生物膜的形成，胆汁和酸的耐受性，以及相互作用的E。大肠杆菌菌株与肠上皮细胞的3-D培养物。在这项研究中，我们表明，质粒的性质和组合，以及宿主菌株的背景，对这些现象有影响。我们的数据揭示了染色体外序列在ExPEC表型适应性和多样性中的作用的新见解。
Extra-intestinal pathogenic E. coli (ExPEC), including avian pathogenic E. coli (APEC), pose a considerable threat to both human and animal health, with illness causing substantial economic loss. APEC strain χ7122 (O78∶K80∶H9), containing three large plasmids [pChi7122-1 (IncFIB/FIIA-FIC), pChi7122-2 (IncFII), and pChi7122-3 (IncI2)]; and a small plasmid pChi7122-4 (ColE2-like), has been used for many years as a model strain to study the molecular mechanisms of ExPEC pathogenicity and zoonotic potential. We previously sequenced and characterized the plasmid pChi7122-1 and determined its importance in systemic APEC infection; however the roles of the other pChi7122 plasmids were still ambiguous. Herein we present the sequence of the remaining pChi7122 plasmids, confirming that pChi7122-2 and pChi7122-3 encode an ABC iron transport system (eitABCD) and a putative type IV fimbriae respectively, whereas pChi7122-4 is a cryptic plasmid. New features were also identified, including a gene cluster on pChi7122-2 that is not present in other E. coli strains but is found in Salmonella serovars and is predicted to encode the sugars catabolic pathways. In vitro evaluation of the APEC χ7122 derivative strains with the three large plasmids, either individually or in combinations, provided new insights into the role of plasmids in biofilm formation, bile and acid tolerance, and the interaction of E. coli strains with 3-D cultures of intestinal epithelial cells. In this study, we show that the nature and combinations of plasmids, as well as the background of the host strains, have an effect on these phenomena. Our data reveal new insights into the role of extra-chromosomal sequences in fitness and diversity of ExPEC in their phenotypes.
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影响因子：
3.1
作者：
Dudley, EG;Abe, C;Nataro, JP
通讯作者：
Nataro, JP
影响因子：
3.6
作者：
Barua, S;Yamashino, T;Ohta, M
通讯作者：
Ohta, M
DOI：
10.1126/science.1180614
发表时间：
2009-10-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chain PS;Grafham DV;Fulton RS;Fitzgerald MG;Hostetler J;Muzny D;Ali J;Birren B;Bruce DC;Buhay C;Cole JR;Ding Y;Dugan S;Field D;Garrity GM;Gibbs R;Graves T;Han CS;Harrison SH;Highlander S;Hugenholtz P;Khouri HM;Kodira CD;Kolker E;Kyrpides NC;Lang D;Lapidus A;Malfatti SA;Markowitz V;Metha T;Nelson KE;Parkhill J;Pitluck S;Qin X;Read TD;Schmutz J;Sozhamannan S;Sterk P;Strausberg RL;Sutton G;Thomson NR;Tiedje JM;Weinstock G;Wollam A;Genomic Standards Consortium Human Microbiome Project Jumpstart Consortium;Detter JC
通讯作者：
Detter JC
影响因子：
9.5
作者：
Berriman, Matt;Rutherford, Kim
通讯作者：
Rutherford, Kim
影响因子：
3.2
作者：
FURUYA, N;KOMANO, T
通讯作者：
KOMANO, T