DNA distortion and specificity in a sequence-specific endonuclease.
DNA distortion and specificity in a sequence-specific endonuclease.
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DOI：
10.1016/j.jmb.2008.08.032
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发表时间：
2008-10-31
影响因子：
5.6
通讯作者：
Horton, Nancy C.
中科院分区：
文献类型：
--
作者：
Babic, Andrea C.;Little, Elizabeth J.;Manohar, Veena M.;Bitinaite, Jurate;Horton, Nancy C.
Five new structures of the Q138F HincII enzyme bound to a total of three different DNA sequences and three different metal ions (Ca2+, Mg2+, and Mn2+) are presented. While previous structures were produced from soaking Ca2+ into pre-formed Q138F HincII/DNA crystals, the new structures are derived from cocrystallization with Ca2+, Mg2+, or Mn2+. The Mn2+ bound structure provides the first view of a product complex of Q138F HincII with cleaved DNA. Binding studies and a crystal structure show how Ca2+ allows trapping of a Q138F HincII complex with noncognate DNA in a catalytically incompetent conformation. Many Q138F HincII/DNA structures show asymmetry, despite the binding of a symmetric substrate by a symmetric enzyme. The various complexes are fit into a model describing the different conformations of the DNA bound enzyme, and show how DNA conformational energetics determine DNA cleavage rates by the Q138F HincII enzyme.
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影响因子：
5.6
作者：
Chen, SF;Gunasekera, A;Berman, HM
通讯作者：
Berman, HM
影响因子：
2.9
作者：
Galburt, EA;Stoddard, BL
通讯作者：
Stoddard, BL
影响因子：
5.6
作者：
Etzkorn, C;Horton, NC
通讯作者：
Horton, NC
影响因子：
15
作者：
Horton, NC;Connolly, BA;Perona, JJ
通讯作者：
Perona, JJ
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL