A reinforced PDMS mold for hot embossing of cyclic olefin polymer in the fabrication of microfluidic chips.
A reinforced PDMS mold for hot embossing of cyclic olefin polymer in the fabrication of microfluidic chips.
复制标题
DOI：
10.1039/d2lc00857b
复制
发表时间：
2022-11-22
期刊：
影响因子：
6.1
通讯作者：
中科院分区：
文献类型：
--
作者：
热压印是一种成本效益高且灵活的制造技术，对于小至50纳米的特征尺寸具有高复制精度。在这里，我们开发了一种用于微流控芯片制造中环烯烃聚合物(COP)片材热压成型的增强型聚二甲基硅氧烷(PDMS)模具，并通过制作用于数字核酸分析中自动样品数字化的芯片来演示该方法。PDMS通过添加包埋粉作为掺杂剂进行硬化，并用铝框约束，以防止在热压过程中横向膨胀。增强的聚二甲基硅氧烷模具表现出优异的热压印性能(180℃，103kPa，5min)，用于微图案COP板材，具有高重复性特征，小至10μm(排气通道宽度)。相反，当从PDMS模具中省略包埋粉或框架时，微观特征不一致和扭曲。CoP芯片是通过热结合有图案的和未有图案的CoP片来组装的。我们在用于人类乳头瘤病毒18型(HPV-18)DNA定量的数字灯检测中测试了用于自动样本数字化的COP芯片的性能。用注射器泵将混合的核酸扩增试剂装入芯片的主通道，然后将溶液自发分成几个室(约0.6nL)，然后用流过芯片的油进行分离。数字LAMP分析在10到1,000拷贝/μL的浓度范围内产生准确的DNA绝对定量。这里提出的策略提供了一种简单、低成本的方法来准备用于热压印的模具，这有助于快速验证微流控设计。为了便于微流控芯片设计的快速验证，开发了一种用于微流控芯片制造中环烯烃聚合物热压成型的增强型PDMS模具。
Hot embossing is a cost-effective and flexible fabrication technology with high replication accuracy for feature sizes as small as 50 nm. Here we develop a reinforced polydimethylsiloxane (PDMS) mold for hot embossing of cyclic olefin polymer (COP) sheets in the fabrication of microfluidic chips and demonstrate the method by fabricating chips for automated sample digitization in digital nucleic acid assays. The PDMS is hardened by adding an investment powder as a dopant and is constrained with an aluminum frame to prevent lateral expansion during hot pressing. The reinforced PDMS mold demonstrated excellent performance in hot embossing (180 °C, 103 kPa, 5 min) for micropatterning COP sheets, with highly reproducible features as small as 10 μm (width of draining channel). In contrast, the microscale features were inconsistent and distorted when omitting either the investment powder or frame from the PDMS mold. COP chips were assembled by thermally bonding patterned and unpatterned COP sheets. We tested the performance of the COP chip for automated sample digitization in a digital LAMP assay used to quantify human papillomavirus-18 (HPV-18) DNA. A mixture of nucleic acid amplification reagents was loaded into the main channel of the chip using a syringe pump, then the solution was spontaneously partitioned into chambers (~0.6 nL), which were then isolated by flowing oil through the chip. The digital LAMP assay produced accurately absolute quantitation of DNA at concentrations ranging from 10 to 1,000 copies/μL. The strategy presented here provides a simple, low-cost method to prepare molds for hot embossing, which facilitates rapid validation of microfluidic designs. A reinforced PDMS mold for hot embossing of cyclic olefin polymer in the fabrication of microfluidic chips was developed, which facilitates the rapid validation of microfluidic designs.
登录
查看更多内容
影响因子：
7.4
作者：
Gu, Pan;Liu, Ke;Chen, Hong;Nishida, Toshikazu;Fan, Z. Hugh
通讯作者：
Fan, Z. Hugh
影响因子：
6.1
作者：
Novak R;Ranu N;Mathies RA
通讯作者：
Mathies RA
影响因子：
7.4
作者：
Heo, Yun Seok;Cabrera, Lourdes M.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
17.1
作者：
Koebley, Sean R.;Mikheikin, Andrey;Reed, Jason
通讯作者：
Reed, Jason
影响因子：
48
作者：
Hindson, Christopher M.;Chevillet, John R.;Briggs, Hilary A.;Gallichotte, Emily N.;Ruf, Ingrid K.;Hindson, Benjamin J.;Vessella, Robert L.;Tewari, Muneesh
通讯作者：
Tewari, Muneesh