Multiple differentiation capacity of STRO-1+/CD146+ PDL mesenchymal progenitor cells.
Multiple differentiation capacity of STRO-1+/CD146+ PDL mesenchymal progenitor cells.
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DOI：
10.1089/scd.2008.0113
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发表时间：
2009-04
影响因子：
4
通讯作者：
Kapila S
中科院分区：
文献类型：
--
作者：
Xu J;Wang W;Kapila Y;Lotz J;Kapila S
尽管可以使用干细胞标记物 STRO-1 和 CD146 从牙周膜 (PDL) 组织中分离间充质祖细胞，但这些细胞中具有分化成多种细胞谱系能力的比例仍有待确定。本研究旨在量化可进行多谱系分化的原代人 PDL 细胞的比例，并比较这些能力相对于骨髓源性间充质干细胞 (MSC) 和亲本 PDL (PPDL) 细胞的大小。使用标记 STRO-1 和 CD146 从 PPDL 细胞中分离 PDL 间充质祖细胞 (PMP)。测定了 PMP、PPDL 和 MSC 在软骨形成、成骨和脂肪形成条件下的集落形成效率和多谱系分化潜力。流式细胞术显示，平均 2.6% 的 PPDL 细胞为 STRO-1+/CD146+，而超过 63% 为 STRO-1-/CD146-。 STRO-1+/CD146+ PMP细胞（19.3%）和MSCs（16.7%）的集落形成效率显着高于PPDL细胞（6.8%）。与未诱导的对应物或诱导的 PPDL 细胞相比，在适当的条件下，PMP 细胞和 MSC 中的软骨特异性基因、成骨细胞分化的早期标记物和脂肪生成标记物显着上调。与这些发现一致，免疫组织化学显示，与未诱导的对照或诱导的 PPDL 细胞相比，在 PMP 和 MSC 培养物中的软骨形成、成骨或脂肪形成条件下，软骨大分子、矿化钙结节和脂质空泡分别大量积累。因此，STRO-1+/CD146+ PMP 细胞表现出与 MSC 相当的多谱系分化能力，并且有可能用于牙周组织和其他组织的再生。
Although mesenchymal progenitor cells can be isolated from periodontal ligament (PDL) tissues using stem cell markers STRO-1 and CD146, the proportion of these cells that have the capacity to differentiate into multiple cell lineages remains to be determined. This study was designed to quantify the proportions of primary human PDL cells that can undergo multi-lineage differentiation, and to compare the magnitude of these capabilities relative to bone marrow-derived mesenchymal stem cells (MSC) and parental PDL (PPDL) cells. PDL mesenchymal progenitor (PMP) cells were isolated from PPDL cells using the markers STRO-1 and CD146. The colony-forming efficiency and multi-lineage differentiation potential of PMP, PPDL, and MSCs under chondrogenic, osteogenic and adipogenic conditions were determined. Flow cytometry revealed that on average 2.6% of PPDL cells were STRO-1+/CD146+, whereas more than 63% were STRO-1-/CD146-. Colony-forming efficiency of STRO-1+/CD146+ PMP cells (19.3%) and MSCs (16.7%) was significantly higher than that of PPDL cells (6.8%). Cartilage specific genes, early markers of osteoblastic differentiation, and adipogenic markers were significantly up-regulated under appropriate conditions in PMP cells and MSCs compared to either their non-induced counterparts or induced PPDL cells. Consistent with these findings, immunohistochemistry revealed substantial accumulation of cartilaginous macromolecules, mineralized calcium nodules, and lipid vacuoles under chondrogenic, osteogenic or adipogenic conditions in PMP and MSC cultures, respectively, compared to non-induced controls or induced PPDL cells. Thus, STRO-1+/CD146+ PMP cells demonstrate multi-lineage differentiation capacity comparable in magnitude to MSCs, and could potentially be utilized for regeneration of the periodontium and other tissues.
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