Comprehensive characterization of four different populations of human mesenchymal stem cells as regards their immune properties, proliferation and differentiation.
Comprehensive characterization of four different populations of human mesenchymal stem cells as regards their immune properties, proliferation and differentiation.
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DOI：
10.3892/ijmm.2014.1821
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发表时间：
2014-09
影响因子：
5.4
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Li X;Bai J;Ji X;Li R;Xuan Y;Wang Y
本研究比较了人骨髓(BM)、脂肪组织(AT)、脐带Wharton凝胶(WJ)和胎盘(PL)4种来源的间充质干细胞(MSCs)，以确定哪种MSCs对植物血凝素诱导的T细胞增殖具有最显著的免疫抑制作用，哪种MSCs具有最高的增殖和分化潜力。采用MSC与T淋巴细胞共培养(混合培养)来确定MSCs是否抑制T细胞的增殖，以及哪种MSCs的抑制能力最强。用RT-PCR和RT-qPCR分析免疫相关基因的表达。用标准方法检测骨髓间充质干细胞的增殖分化潜能。MSC与T细胞共培养后，4种来源的MSCs均能有效抑制丝裂原诱导的T细胞增殖。这是通过可溶因素而不是直接接触抑制发生的。在4个MSCs群体中，WJ-MSC的抑制作用最强。在免疫相关基因上，WJ-MSC的MHC II基因TLR4、TLR3、JAG1、NOTCH2和NOTCH3的表达最弱。比较细胞增殖能力，WJ-MSCs生长速度最快，AT-MSCs次之，PL-MSCs次之，BM-MSCs次之。在分化潜能方面，WJ-MSCs的成骨能力最强，其次是PL、AT和BM-MSC。AT-MSC成脂能力最强，其次是WJ-MSCs、BM-MSCs和PL-MSCs。这些数据表明WJ-MSCs具有最强的免疫调节和免疫抑制能力。根据这些观察，我们认为当需要免疫抑制作用时，WJ-MSCs是用于免疫细胞治疗的最有吸引力的细胞群。
In the present study, we compared mesenchymal stem cells (MSCs) derived from 4 different sources, human bone marrow (BM), adipose tissue (AT), umbilical cord Wharton’s Jelly (WJ) and the placenta (PL), in order to determine which population of MSCs displayed the most prominent immunosuppressive effects on phytohemagglutinin-induced T cell proliferation, and which one had the highest proliferative and differentiation potential. MSC and T lymphocyte co-culture (mixed culture) was used to determine whether the MSCs inhibit T cell proliferation, as well as which population of MSCs has the strongest inhibitory ability. The expression of immune-related genes was analyzed by RT-PCR and RT-qPCR. The proliferation and differentiation potential of the MSCs were determined using standard methods. Following MSC and T cell co-culture, mitogen-induced T cell proliferation was effectively suppressed by all 4 populations of MSCs. This occurred through soluble factors rather than direct contact inhibition. Among the 4 populations of MSCs, the WJ-MSC has the strongest suppression effects. On immune related genes, WJ-MSC has the weakest expression of MHC II genes, TLR4, TLR3, JAG1, NOTCH2 and NOTCH3. To compare the proliferation potential, WJ-MSCs showed the most rapid growth rate followed by the AT-, PL- and BM-MSCs. As regards differentiation potential, the WJ-MSCs had the strongest osteogenetic ability followed by PL, AT and BM-MSC. AT-MSC has the strongest adipogenetic ability followed by the WJ-, BM- and PL-MSCs. These data indicated that the WJ-MSCs had the strongest immunomodulatory and immunosuppressive potential. In light of these observations, we suggest that WJ-MSCs are the most attractive cell population for use in immune cellular therapy when immunosuppressive action is required.
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影响因子：
20.3
作者：
Beltrami, Antonio R.;Cesselli, Daniela;Beltrami, Carlo A.
通讯作者：
Beltrami, Carlo A.
影响因子：
2.1
作者：
Dazzi, Francesco;Krampera, Mauro
通讯作者：
Krampera, Mauro
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
6.2
作者：
Larsen, Kenneth H.;Frederiksen, Casper M.;Kassem, Moustapha
通讯作者：
Kassem, Moustapha
影响因子：
5.2
作者：
Baksh, Dolores;Yao, Raphael;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.