Ultrasound-stimulated drug delivery for treatment of residual disease after incomplete resection of head and neck cancer.
Ultrasound-stimulated drug delivery for treatment of residual disease after incomplete resection of head and neck cancer.
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DOI：
10.1016/j.ultrasmedbio.2013.11.002
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发表时间：
2014-04
影响因子：
2.9
通讯作者：
Hoyt, Kenneth
中科院分区：
文献类型：
--
作者：
Sorace, Anna G.;Korb, Melissa;Warram, Jason M.;Umphrey, Heidi;Zinn, Kurt R.;Rosenthal, Eben;Hoyt, Kenneth
关键词：
局部超声触发的微泡可以改善肿瘤药物的传递和保留。该策略被称为us刺激药物递送，应用于头颈癌（HNC）手术后肿瘤切除模型。裸鼠（N = 24）植入荧光素酶阳性HNC鳞状细胞癌（SCC）的腹部，分别进行不同程度的手术切除（0%、50%和100%）。手术后，动物在第4,7和10天接受西妥昔单抗- irdye单独辅助治疗，西妥昔单抗- irdye联合us刺激给药或生理盐水注射（对照组）。在第0、4、7、10、14、17天用体内荧光成像系统评估肿瘤给药情况，同时在同一时间点用体内生物发光成像系统评估肿瘤生存能力。肿瘤卡尺测量每周进行两次，持续数天。光学成像显示，在50%肿瘤切除组中，在第10天（荧光峰值日），us刺激给药导致西妥昔单抗给药量比单独给药显著增加（p = 0.03）。与单用药物组相比，所有接受us刺激药物传递加药物组的肿瘤生存能力均下降。在不同程度的手术切除后，这项新的研究表明，当结合us刺激的药物递送时，残余癌细胞的药物摄取得到了积极的改善。
Microbubbles triggered with localized ultrasound (US) can improve tumor drug delivery and retention. Termed US-stimulated drug delivery, this strategy was applied to head and neck cancer (HNC) in a post-surgical tumor resection model. Nude athymic mice (N = 24) were implanted in the flank with luciferase-positive HNC squamous cell carcinoma (SCC) and underwent various degrees of surgical tumor resection (0%, 50% or 100%). Following surgery, animals received adjuvant therapy with cetuximab-IRDye alone, cetuximab-IRDye in combination with US-stimulated drug delivery, or saline injections (control) on days 4, 7, and 10. Tumor drug delivery was assessed on days 0, 4, 7, 10, 14, and 17 with an in vivo fluorescence imaging system, while tumor viability was evaluated at the same time points with in vivo bioluminescence imaging. Tumor caliper measurements occurred two times per week for days. Optical imaging demonstrated that in the 50% tumor resection group, US-stimulated drug delivery resulted in a significant increase in cetuximab delivery compared to drug alone on day 10 (day of peak fluorescence) (p = 0.03). Tumor viability decreased in all groups receiving US-stimulated drug delivery plus drug compared to the drug alone group. After various degrees of surgical resection, this novel study demonstrates positive improvements in drug uptake in the residual cancer cells when combined with US-stimulated drug delivery.
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影响因子：
11.2
作者：
McDannold N;Arvanitis CD;Vykhodtseva N;Livingstone MS
通讯作者：
Livingstone MS
影响因子：
2.6
作者：
Day, Kristine E.;Beck, Lauren N.;Deep, Nicholas L.;Kovar, Joy;Zinn, Kurt R.;Rosenthal, Eben L.
通讯作者：
Rosenthal, Eben L.
影响因子：
4.9
作者：
Escoffre, J. M.;Piron, J.;Bouakaz, A.
通讯作者：
Bouakaz, A.
影响因子：
2.4
作者：
Caskey, Charles F.;Stieger, Susanne M.;Ferrara, Katherine W.
通讯作者：
Ferrara, Katherine W.
DOI：
10.1073/pnas.0604318103
发表时间：
2006-08-01
影响因子：
11.1
作者：
Kinoshita, Manabu;McDannold, Nathan;Hynynen, Kullervo
通讯作者：
Hynynen, Kullervo