Fluorescently labeled therapeutic antibodies for detection of microscopic melanoma.
Fluorescently labeled therapeutic antibodies for detection of microscopic melanoma.
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DOI：
10.1002/lary.24102
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发表时间：
2013-11
期刊：
影响因子：
2.6
通讯作者：
Rosenthal, Eben L.
中科院分区：
文献类型：
--
作者：
Day, Kristine E.;Beck, Lauren N.;Deep, Nicholas L.;Kovar, Joy;Zinn, Kurt R.;Rosenthal, Eben L.
在手术切除黑色素瘤的过程中检测显微疾病仍然是一个巨大的挑战。为了评估实时光学成像对显微癌症的可视化，我们评估了FDA批准的三种治疗性单抗。将预期的基础科学黑色素瘤细胞系(A375和SKMEL5)异种移植到免疫缺陷小鼠的耳朵中。将贝伐单抗、Panitumab、tocilizumab或非特异性抗体与近红外(NIR)荧光探针(IRDye800CW)共价连接并系统注射。原发肿瘤被成像，然后在荧光引导下使用SPY进行切除，SPY是一种用于整形和重建手术的近红外成像系统，用于评估血流灌注。小鼠也被用珍珠脉冲小动物成像仪成像，这是一种为IRDye800CW设计的近红外成像系统。切除后，对小组织碎片进行荧光成像，随后与组织学对照证实肿瘤的存在。与Ig G-IRDye800CW相比，所有荧光标记的治疗性单抗都可以根据肿瘤与背景的比率(TBR)充分区分肿瘤和正常组织。在系列成像上，Panitumab对SPY和PEARE的TBR最高(3.8和6.6)。当用于指导切除时，抗体-染料结合物使用SPY产生的TBR范围为1.3-2.2(平均=1.6)，使用珍珠产生的TBR为1.9-6.3(平均=2.7)。无论是成像方式还是细胞系(单因素方差分析)，抗体之间均无显著差异。我们的数据表明，FDA批准的抗体可能是术中荧光检测黑色素瘤的合适靶向试剂。不适用
Detection of microscopic disease during surgical resection of melanoma remains a significant challenge. To assess real-time optical imaging for visualization of microscopic cancer, we evaluated three FDA-approved therapeutic monoclonal antibodies. Prospective, basic science Melanoma cell lines (A375 and SKMEL5) were xenografted into the ears of immunodeficient mice. Bevacizumab, panitumumab, tocilizumab, or a non-specific IgG were covalently linked to a near-infrared (NIR) fluorescent probe (IRDye800CW) and systemically injected. Primary tumors were imaged and then resected under fluorescent guidance using the SPY, an NIR imaging system used in plastic and reconstructive surgeries to evaluate perfusion. Mice were also imaged with the Pearl Impulse small animal imager, an NIR imaging system designed for use with IRDye800CW. Post-resection, small tissue fragments were fluorescently imaged and presence of tumor subsequently confirmed by correlation with histology. All fluorescently-labeled therapeutic monoclonal antibodies could adequately delineate tumor from normal tissue based on tumor-to-background ratios (TBR) compared to IgG-IRDye800CW. On serial imaging, panitumumab achieved the highest TBRs with both SPY and Pearl (3.8 and 6.6). When used to guide resections, the antibody-dye conjugates generated TBRs in the range of 1.3-2.2 (average=1.6) using the SPY and 1.9-6.3 (average=2.7) using the Pearl. There was no significant difference amongst the antibodies with either imaging modality or cell line (one-way ANOVA). Our data suggests that FDA approved antibodies may be suitable targeting agents for the intraoperative fluorescent detection of melanoma. N/A
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影响因子：
3.1
作者：
Marshall, Milton V.;Draney, Daniel;Sevick-Muraca, Eva M.;Olive, D. Michael
通讯作者：
Olive, D. Michael
影响因子：
3.1
作者：
Keereweer, Stijn;Kerrebijn, Jeroen D. F.;van Driel, Pieter B. A. A.;Xie, Bangwen;Kaijzel, Eric L.;Snoeks, Thomas J. A.;Que, Ivo;Hutteman, Merlijn;van der Vorst, Joost R.;Mieog, J. Sven D.;Vahrmeijer, Alexander L.;van de Velde, Cornelis J. H.;de Jong, Robert J. Baatenburg;Lowik, Clemens W. G. M.
通讯作者：
Lowik, Clemens W. G. M.
临床翻译的二氧化硅纳米颗粒作为以癌为标志性的探针进行图像引导的手术和干预措施。
DOI：
10.1039/c2ib20174g
发表时间：
2013-01
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Bradbury MS;Phillips E;Montero PH;Cheal SM;Stambuk H;Durack JC;Sofocleous CT;Meester RJ;Wiesner U;Patel S
通讯作者：
Patel S
影响因子：
3.7
作者：
Brouwer, Oscar R.;Klop, W. Martin C.;van Leeuwen, Fijs W. B.
通讯作者：
van Leeuwen, Fijs W. B.
影响因子：
3
作者：
OBRIEN, CJ;COATES, AS;MCCARTHY, WH
通讯作者：
MCCARTHY, WH