The Pupa Stage Is the Most Sensitive to Hypoxia in Drosophila melanogaster.
The Pupa Stage Is the Most Sensitive to Hypoxia in Drosophila melanogaster.
复制标题
果蝇蛹期对缺氧最敏感。
DOI：
10.3390/ijms25020710
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发表时间：
2024-01-05
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
缺氧不仅在多种疾病中起着关键作用;它还影响细胞，组织和器官的生长和发育。为了确定新的缺氧相关机制参与细胞和组织的生长，研究一个精确的缺氧敏感的时间窗口可能是一种有效的方法。黑腹果蝇一直是研究各种条件的有用模式生物，我们在这项研究中的重点是果蝇的生活史阶段，以探讨其缺氧敏感性。常氧条件下，在蛹期用4%O_2处理3、2和1d/s，羽化率分别为6.1%、66.7%和96.4%，在整个蛹期保持4%O_2，此处理方案是致死的。令人惊讶的是，当我们的低氧适应苍蝇谁通常生活在4%的O2处理与4%的O2在蛹阶段，没有苍蝇羽化。在蛹期，蛹形成后第2和第3天的蛹在处理2天时分别表现出12.5 ± 8.5%和23.6 ± 1.6%的羽化率，但当处理3天时，这是完全致死的。我们的结论是，pupelia，在2天的APF和持续时间的至少2天，是最敏感的缺氧。我们的数据从我们的缺氧适应苍蝇清楚地表明，表观遗传因素在蛹阶段的缺氧敏感性发挥了关键作用。
Hypoxia not only plays a critical role in multiple disease conditions; it also influences the growth and development of cells, tissues and organs. To identify novel hypoxia-related mechanisms involved in cell and tissue growth, studying a precise hypoxia-sensitive time window can be an effective approach. Drosophila melanogaster has been a useful model organism for studying a variety of conditions, and we focused in this study on the life cycle stages of Drosophila to investigate their hypoxia sensitivity. When normoxia-grown flies were treated with 4% O2 at the pupa stage for 3, 2 and 1 day/s, the eclosion rates were 6.1%, 66.7% and 96.4%, respectively, and, when 4% O2 was kept for the whole pupa stage, this regimen was lethal. Surprisingly, when our hypoxia-adapted flies who normally live in 4% O2 were treated with 4% O2 at the pupa stage, no fly eclosed. Within the pupa stage, the pupae at 2 and 3 days after pupae formation (APF), when treated for 2 days, demonstrated 12.5 ± 8.5% and 23.6 ± 1.6% eclosion, respectively, but this was completely lethal when treated for 3 days. We conclude that pupae, at 2 days APF and for a duration of a minimum of 2 days, were the most sensitive to hypoxia. Our data from our hypoxia-adapted flies clearly indicate that epigenetic factors play a critical role in pupa-stage hypoxia sensitivity.
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影响因子：
16.6
作者：
Obata F;Fons CO;Gould AP
通讯作者：
Gould AP
热事件对飞蛾不同发育阶段的影响：越接近成虫阶段，繁殖产出越少
影响因子：
4.6
作者：
Zhang W;Chang XQ;Hoffmann A;Zhang S;Ma CS
通讯作者：
Ma CS
DOI：
10.1073/pnas.88.13.5680
发表时间：
1991-07-01
影响因子：
11.1
作者：
SEMENZA, GL;NEJFELT, MK;ANTONARAKIS, SE
通讯作者：
ANTONARAKIS, SE
DOI：
10.1152/ajpregu.2001.280.5.r1555
发表时间：
2001-05-01
影响因子：
2.8
作者：
Douglas, RM;Xu, T;Haddad, GG
通讯作者：
Haddad, GG
影响因子：
8.8
作者：
通讯作者：
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