Comparative Transcriptomics of Malaria Mosquito Testes: Function, Evolution, and Linkage.
Comparative Transcriptomics of Malaria Mosquito Testes: Function, Evolution, and Linkage.
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DOI：
10.1534/g3.117.040089
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发表时间：
2017-04-03
期刊：
影响因子：
--
通讯作者：
White BJ
中科院分区：
文献类型：
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作者：
Cassone BJ;Kay RG;Daugherty MP;White BJ
睾丸偏向基因进化迅速，在早期物种之间生殖隔离的建立、固化和维持中起重要作用。冈比亚按蚊复合体是撒哈拉以南非洲特有的一组至少8种同构蚊子，是探索睾丸偏向基因进化的极好系统。在这个群体中，睾丸是多样化过程中的一个重要组织，因为物种之间的杂交会导致不育的杂交雄性，但完全能生育的雌性。我们对A. gambiae和A. merus尸体和睾丸，以探索组织和物种特异性的基因表达模式。我们的数据为男性生殖系中X连锁基因的转录抑制提供了支持，这可能会驱动X染色体的去男性化。睾丸偏向基因主要在细胞分化中发挥作用，并显示出许多有趣的模式，表明它们的快速进化，包括升高的dN/dS值，低进化保守性，现有参考基因组中的注释差，以及物种间差异表达的可能性高。
Testes-biased genes evolve rapidly and are important in the establishment, solidification, and maintenance of reproductive isolation between incipient species. The Anopheles gambiae complex, a group of at least eight isomorphic mosquito species endemic to Sub-Saharan Africa, is an excellent system to explore the evolution of testes-biased genes. Within this group, the testes are an important tissue in the diversification process because hybridization between species results in sterile hybrid males, but fully fertile females. We conducted RNA sequencing of A. gambiae and A. merus carcass and testes to explore tissue- and species-specific patterns of gene expression. Our data provides support for transcriptional repression of X-linked genes in the male germline, which likely drives demasculinization of the X chromosome. Testes-biased genes predominately function in cellular differentiation and show a number of interesting patterns indicative of their rapid evolution, including elevated dN/dS values, low evolutionary conservation, poor annotation in existing reference genomes, and a high likelihood of differential expression between species.
DOI：
10.1155/2012/708980
发表时间：
2012
期刊：
International journal of evolutionary biology
影响因子：
--
作者：
Dubruille R;Marais GA;Loppin B
通讯作者：
Loppin B