Physiological and molecular responses of invasive cyanobacterium Raphidiopsis raciborskii to ambient phosphorus deficiency
Physiological and molecular responses of invasive cyanobacterium Raphidiopsis raciborskii to ambient phosphorus deficiency
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侵入性蓝藻Raphidiopsis raciborskii对环境缺磷的生理和分子反应
DOI：
10.1007/s00343-022-1314-z
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发表时间：
2022-07
影响因子：
1.6
通讯作者：
Zhongxing Wu
中科院分区：
文献类型：
--
作者：
Junqiong Shi;Shuhan He;Lu Zhao;Lulu Ji;Songqi Yang;Zhongxing Wu
当环境磷（P）浓度非常低时，Raphidiopsis raciborskii 会引起有害的蓝藻水华，因此需要更好地表征其适应 P 的生理和分子机制。比较了有和没有无机磷生长的 R. raciborskii FACHB 1496 菌株的生长、叶绿素荧光、碱性磷酸酶和直接参与磷同化的基因表达。比生长速率 (μ)、叶绿素 a 和六个荧光参数（最小荧光 (F0)、最大荧光 (Fm)、最大可变荧光 (Fv)、每个 RC (ET0/RC) 的电子传输通量（超出 QA）、量子PSII 中电子传输的产率 (ØE0)，以及 R. raciborskii 中来自俘获激子的电子移动到超过 QA− (ψ0)) 的电子传输链的概率显着降低，以响应实验性的 P 缺陷。相比之下，J 步 (VJ) 的相对可变荧光、每个 RC (TR0/RC) 的捕获能量通量（导致 QA 减少）和碱性磷酸酶活性显着增加。此外，缺磷条件下，涉及碱性磷酸酶（phoA1和phoA2）、高亲和力无机磷转运蛋白（pstS1）、磷酸盐转运蛋白和代谢（phnD和phnM）以及核苷酸酶（nucH）的基因表达显着上调。然而，在缺磷条件下重新补充磷后，生理和分子反应迅速恢复。我们的结果强调，R. raciborskii 可以执行协调且复杂的细胞和生理反应来应对磷缺乏，这反映了 R. raciborskii 响应环境磷波动的多方面机制。
Raphidiopsis raciborskii can cause harmful cyanobacterial blooms when concentrations of environmental phosphorus (P) are very low, thus the physiological and molecular mechanisms involved in the acclimation to P need to be characterized better. The growth, chlorophyll fluorescence, alkaline phosphatase, and expression of genes directly involved in P assimilation were compared in the R. raciborskii FACHB 1496 strain grown with and without inorganic P. The specific growth rate (μ), Chl a, and six fluorescence parameters (minimal fluorescence (F0), maximal fluorescence (Fm), maximal variable fluorescence (Fv), electron transport flux (further than QA) per RC (ET0/RC), quantum yield of the electron transport in PSII (ØE0), and the probability that an electron from a trapped exciton is moved into the electron transport chain beyond QA− (ψ0)) markedly decreased in R. raciborskii in response to experimental P-deficiency. In contrast, the relative variable fluorescence at the J-step (VJ), trapped energy flux (leading to QA reduction) per RC (TR0/RC), and alkaline phosphatase activity significantly increased. In addition, gene expressions involved in the alkaline phosphatase (phoA1 and phoA2), high-affinity inorganic P transporter (pstS1), phosphonate transporter and metabolism (phnD and phnM), and nucleotidase (nucH) were significantly upregulated under P deficiency. However, physiological and molecular responses were resumed rapidly after P re-supplementation following P-deficient conditions. Our results highlight that R. raciborskii can perform coordinated and complex cellular and physiological responses to cope with P deficiency, reflecting R. raciborskii’s multi-faceted machinery to respond to environmental P fluctuations.
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影响因子：
3.7
作者：
Stucken K;John U;Cembella A;Murillo AA;Soto-Liebe K;Fuentes-Valdés JJ;Friedel M;Plominsky AM;Vásquez M;Glöckner G
通讯作者：
Glöckner G
影响因子：
4.4
作者：
Sinha R;Pearson LA;Davis TW;Muenchhoff J;Pratama R;Jex A;Burford MA;Neilan BA
通讯作者：
Neilan BA
影响因子：
1.8
作者：
M. Saker;D. Griffiths
通讯作者：
M. Saker;D. Griffiths
影响因子：
1.5
作者：
O. A. Vershinina;L. Znamenskaya
通讯作者：
O. A. Vershinina;L. Znamenskaya
通过生理学和转录组分析揭示磷酸甘油 (DOP) 对甲藻双足多甲藻的影响
影响因子：
5.9
作者：
Yang Yanjun;Shi Junqiong;Jia Yunlu;Bai Fang;Yang Songqi;Mi Wenmei;He Shuhan;Wu Zhongxing
通讯作者：
Wu Zhongxing