Unveiling the impact of glycerol phosphate (DOP) in the dinoflagellate Peridinium bipes by physiological and transcriptomic analysis
Unveiling the impact of glycerol phosphate (DOP) in the dinoflagellate Peridinium bipes by physiological and transcriptomic analysis
复制标题
通过生理学和转录组分析揭示磷酸甘油 (DOP) 对甲藻双足多甲藻的影响
DOI：
10.1186/s12302-020-00317-6
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发表时间：
2020-03
影响因子：
5.9
通讯作者：
Wu Zhongxing
中科院分区：
文献类型：
--
作者：
Yang Yanjun;Shi Junqiong;Jia Yunlu;Bai Fang;Yang Songqi;Mi Wenmei;He Shuhan;Wu Zhongxing
背景
在浮游植物面临溶解无机磷(DIP)缺乏的情况下，利用溶解有机磷(DOP)的能力对浮游植物的生存和竞争至关重要。然而，淡水甲藻Periddium Bipes对磷的有效性相对较少受到关注，浮游植物利用甘油磷酸盐的效率很少被研究，其调控分子机制尚不清楚。
结果
在本研究中，淡水甲藻Periddium Bipes的培养设置在119培养基(+DIP)、去DIP的119培养基(无P)和β-甘油-磷酸盐替代-DIP(+DOP)培养基上。用转录转录测序技术分析基因表达情况。DOP处理组细胞生长速度与DIP组相似，但叶绿素a荧光参数RC/CS0、ABS/CS0、TR0/CS0、ET0/CS0和RE0/CS0在DOP组显著降低。转录组分析表明，与DIP组相比，DOP组参与光合作用的基因包括PSBA、PSBB、PSBC、PSBD、PSAA和PSAB表达下调。甘油-3-磷酸脱氢酶和甘油醛-3-磷酸脱氢酶，而不是碱性磷酸酶，负责β-甘油磷酸。DOP组细胞间糖异生代谢明显改变。此外，与DIP处理相比，DOP处理组与DIP处理组相比，参与P.Bipes的ATP合成酶、TCA循环、氧化磷酸化、脂肪酸代谢和氨基酸代谢的基因显著上调。
结论
这些结果表明，β-甘油磷酸盐能够影响两足鱼的光合作用和代谢，这为深入了解三足鱼的磷生理提供了依据。在不同种类的甲藻和其他浮游植物中，β-甘油磷酸和其他DOP的使用机制是不同的。减少倾角可能比减少DOP更有效地控制水华。
Background
The ability to use dissolved organic phosphorus (DOP) is important for survival and competition when phytoplankton are faced with scarcity of dissolved inorganic phosphorus (DIP). However, phosphorus availability to the freshwater dinoflagellate Peridinium bipes has received relatively little attention, the efficiency of glycerol phosphate use by phytoplankton has rarely been investigated, and the regulatory molecular mechanisms remain unclear.
Result
In the present study, cultures of the freshwater dinoflagellate Peridinium bipes were set up in 119 medium (+DIP), DIP-depleted 119 medium (P-free), and β-glycerol phosphate-replacing-DIP medium (+DOP). Gene expression was analyzed using transcriptomic sequencing. The growth rate of cells in DOP treatment group was similar to that in DIP group, but chlorophyll a fluorescence parameters RC/CS0, ABS/CS0, TR0/CS0, ET0/CS0 and RE0/CS0 markedly decreased in the DOP group. Transcriptomic analysis revealed that genes involved in photosynthesis, including psbA, psbB, psbC, psbD, psaA and psaB, were downregulated in the DOP group relative to the DIP group. Glycerol-3-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase, rather than alkaline phosphatase, were responsible for β-glycerol phosphate use. Intercellular gluconeogenesis metabolism was markedly changed in the DOP group. In addition, genes involved in ATP synthases, the TCA cycle, oxidative phosphorylation, fatty acid metabolism and amino acid metabolism in P. bipes were significantly upregulated in the DOP group compared with the DIP treatment.
Conclusions
These findings suggested that β-glycerol phosphate could influence the photosynthesis and metabolism of P. bipes, which provided a comprehensive understanding of the phosphorus physiology of P. bipes. The mechanisms underlying the use of β-glycerol phosphate and other DOPs are different in different species of dinoflagellates and other phytoplankton. DIP reduction may be more effective in controlling the bloom of P. bipes than DOP reduction.
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